Image-guided cardiac cell delivery using high-resolution small-animal ultrasound

Martin G Rodriguez-Porcel, Olivier Gheysens, Ian Y. Chen, Joseph C. Wu, Sanjiv Sam Gambhir

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Open-chest cardiac injection is the preferred delivery method for cardiac gene and stem cell therapy in small animals, but it is invasive and the operator is unable to see the actual delivery site. High-resolution ultrasound has recently been developed for small-animal imaging. We tested the hypothesis that image-guided cardiac cell delivery using high-resolution ultrasound guidance is feasible and reproducible. Sprague-Dawley rats (n = 11) were imaged using high-resolution ultrasound, and stably transfected cardiomyoblasts (plasmid-CMV-firefly luciferase) were injected into the anterior cardiac wall under ultrasound guidance (parasternal long-axis view), using a 28-gauge needle. After injection, bioluminescence imaging was performed using a cooled charged-coupled camera. Injection was successful in all animals and was associated with no mortality. The signal detected was positively correlated with the amount of cells transplanted (R2 = 0.94, P = 0.03) and highly correlated with ex vivo assays (R2 = 0.82). In addition, the optical signal could be followed longitudinally using bioluminescence imaging. Ultrasound image-guided cardiac cell delivery is an effective, safe, and reproducible way to perform cell delivery to a specific myocardial region and can be combined with assessment of cardiac function. We are confident that the use of these technologies will play a significant role in the future of gene and cell therapy.

Original languageEnglish (US)
Pages (from-to)1142-1147
Number of pages6
JournalMolecular Therapy
Volume12
Issue number6
DOIs
StatePublished - Dec 2005

Fingerprint

Cell- and Tissue-Based Therapy
Injections
Firefly Luciferases
Genetic Therapy
Needles
Sprague Dawley Rats
Plasmids
Thorax
Stem Cells
Technology
Mortality
Genes

Keywords

  • Animal models
  • Bioluminescence
  • Molecular imaging
  • Myocardium
  • Ultrasound

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Image-guided cardiac cell delivery using high-resolution small-animal ultrasound. / Rodriguez-Porcel, Martin G; Gheysens, Olivier; Chen, Ian Y.; Wu, Joseph C.; Gambhir, Sanjiv Sam.

In: Molecular Therapy, Vol. 12, No. 6, 12.2005, p. 1142-1147.

Research output: Contribution to journalArticle

Rodriguez-Porcel, Martin G ; Gheysens, Olivier ; Chen, Ian Y. ; Wu, Joseph C. ; Gambhir, Sanjiv Sam. / Image-guided cardiac cell delivery using high-resolution small-animal ultrasound. In: Molecular Therapy. 2005 ; Vol. 12, No. 6. pp. 1142-1147.
@article{ca1bd1ef78e24cb1a51845e9f057fd5f,
title = "Image-guided cardiac cell delivery using high-resolution small-animal ultrasound",
abstract = "Open-chest cardiac injection is the preferred delivery method for cardiac gene and stem cell therapy in small animals, but it is invasive and the operator is unable to see the actual delivery site. High-resolution ultrasound has recently been developed for small-animal imaging. We tested the hypothesis that image-guided cardiac cell delivery using high-resolution ultrasound guidance is feasible and reproducible. Sprague-Dawley rats (n = 11) were imaged using high-resolution ultrasound, and stably transfected cardiomyoblasts (plasmid-CMV-firefly luciferase) were injected into the anterior cardiac wall under ultrasound guidance (parasternal long-axis view), using a 28-gauge needle. After injection, bioluminescence imaging was performed using a cooled charged-coupled camera. Injection was successful in all animals and was associated with no mortality. The signal detected was positively correlated with the amount of cells transplanted (R2 = 0.94, P = 0.03) and highly correlated with ex vivo assays (R2 = 0.82). In addition, the optical signal could be followed longitudinally using bioluminescence imaging. Ultrasound image-guided cardiac cell delivery is an effective, safe, and reproducible way to perform cell delivery to a specific myocardial region and can be combined with assessment of cardiac function. We are confident that the use of these technologies will play a significant role in the future of gene and cell therapy.",
keywords = "Animal models, Bioluminescence, Molecular imaging, Myocardium, Ultrasound",
author = "Rodriguez-Porcel, {Martin G} and Olivier Gheysens and Chen, {Ian Y.} and Wu, {Joseph C.} and Gambhir, {Sanjiv Sam}",
year = "2005",
month = "12",
doi = "10.1016/j.ymthe.2005.07.532",
language = "English (US)",
volume = "12",
pages = "1142--1147",
journal = "Molecular Therapy",
issn = "1525-0016",
publisher = "Nature Publishing Group",
number = "6",

}

TY - JOUR

T1 - Image-guided cardiac cell delivery using high-resolution small-animal ultrasound

AU - Rodriguez-Porcel, Martin G

AU - Gheysens, Olivier

AU - Chen, Ian Y.

AU - Wu, Joseph C.

AU - Gambhir, Sanjiv Sam

PY - 2005/12

Y1 - 2005/12

N2 - Open-chest cardiac injection is the preferred delivery method for cardiac gene and stem cell therapy in small animals, but it is invasive and the operator is unable to see the actual delivery site. High-resolution ultrasound has recently been developed for small-animal imaging. We tested the hypothesis that image-guided cardiac cell delivery using high-resolution ultrasound guidance is feasible and reproducible. Sprague-Dawley rats (n = 11) were imaged using high-resolution ultrasound, and stably transfected cardiomyoblasts (plasmid-CMV-firefly luciferase) were injected into the anterior cardiac wall under ultrasound guidance (parasternal long-axis view), using a 28-gauge needle. After injection, bioluminescence imaging was performed using a cooled charged-coupled camera. Injection was successful in all animals and was associated with no mortality. The signal detected was positively correlated with the amount of cells transplanted (R2 = 0.94, P = 0.03) and highly correlated with ex vivo assays (R2 = 0.82). In addition, the optical signal could be followed longitudinally using bioluminescence imaging. Ultrasound image-guided cardiac cell delivery is an effective, safe, and reproducible way to perform cell delivery to a specific myocardial region and can be combined with assessment of cardiac function. We are confident that the use of these technologies will play a significant role in the future of gene and cell therapy.

AB - Open-chest cardiac injection is the preferred delivery method for cardiac gene and stem cell therapy in small animals, but it is invasive and the operator is unable to see the actual delivery site. High-resolution ultrasound has recently been developed for small-animal imaging. We tested the hypothesis that image-guided cardiac cell delivery using high-resolution ultrasound guidance is feasible and reproducible. Sprague-Dawley rats (n = 11) were imaged using high-resolution ultrasound, and stably transfected cardiomyoblasts (plasmid-CMV-firefly luciferase) were injected into the anterior cardiac wall under ultrasound guidance (parasternal long-axis view), using a 28-gauge needle. After injection, bioluminescence imaging was performed using a cooled charged-coupled camera. Injection was successful in all animals and was associated with no mortality. The signal detected was positively correlated with the amount of cells transplanted (R2 = 0.94, P = 0.03) and highly correlated with ex vivo assays (R2 = 0.82). In addition, the optical signal could be followed longitudinally using bioluminescence imaging. Ultrasound image-guided cardiac cell delivery is an effective, safe, and reproducible way to perform cell delivery to a specific myocardial region and can be combined with assessment of cardiac function. We are confident that the use of these technologies will play a significant role in the future of gene and cell therapy.

KW - Animal models

KW - Bioluminescence

KW - Molecular imaging

KW - Myocardium

KW - Ultrasound

UR - http://www.scopus.com/inward/record.url?scp=28444446265&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=28444446265&partnerID=8YFLogxK

U2 - 10.1016/j.ymthe.2005.07.532

DO - 10.1016/j.ymthe.2005.07.532

M3 - Article

VL - 12

SP - 1142

EP - 1147

JO - Molecular Therapy

JF - Molecular Therapy

SN - 1525-0016

IS - 6

ER -