Image cytophotometric DNA analysis of atypical hyperplasias and intraductal carcinomas of the breast

J. D. Crissman, Daniel W Visscher, J. Kubus

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

With the use of image analysis, DNA content was quantified on paraffin-embedded tissue sections of 25 atypical hyperplasias and 35 intraductal carcinomas of the breast by comparison of integrated gray levels of Feulgen-stained control and ductal cell nuclei. The mean full-peak (G0/G1) control cell DNA histogram coefficient of variation was 5.5%. DNA aneuploidy was more common in intraductal carcinomas compared with atypical hyperplasias (71% of intraductal carcinomas vs 36% of atypical hyperplasias) and correlated with a lack of cytologic (nuclear) and architectural differentiation (63% moderate vs 93% poor and 38% cribriform vs 82% solid). In addition, multiple DNA stemlines were observed in 40% of intraductal carcinomas. We conclude that (1) some atypical hyperplasias demonstrate abnormal DNA content consistent with neoplastic transformation, (2) aggressive forms of intraductal carcinoma are more frequently associated with DNA content abnormalities, and (3) frequent DNA stemline heterogeneity in intraductal carcinoma supports the hypothesis that multiple genetic events occur in the development of mammary intraepithelial neoplasia.

Original languageEnglish (US)
Pages (from-to)1249-1253
Number of pages5
JournalArchives of Pathology and Laboratory Medicine
Volume114
Issue number12
StatePublished - 1990
Externally publishedYes

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Carcinoma, Intraductal, Noninfiltrating
Hyperplasia
Breast
DNA
Aneuploidy
Cell Nucleus
Paraffin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

Cite this

Image cytophotometric DNA analysis of atypical hyperplasias and intraductal carcinomas of the breast. / Crissman, J. D.; Visscher, Daniel W; Kubus, J.

In: Archives of Pathology and Laboratory Medicine, Vol. 114, No. 12, 1990, p. 1249-1253.

Research output: Contribution to journalArticle

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abstract = "With the use of image analysis, DNA content was quantified on paraffin-embedded tissue sections of 25 atypical hyperplasias and 35 intraductal carcinomas of the breast by comparison of integrated gray levels of Feulgen-stained control and ductal cell nuclei. The mean full-peak (G0/G1) control cell DNA histogram coefficient of variation was 5.5{\%}. DNA aneuploidy was more common in intraductal carcinomas compared with atypical hyperplasias (71{\%} of intraductal carcinomas vs 36{\%} of atypical hyperplasias) and correlated with a lack of cytologic (nuclear) and architectural differentiation (63{\%} moderate vs 93{\%} poor and 38{\%} cribriform vs 82{\%} solid). In addition, multiple DNA stemlines were observed in 40{\%} of intraductal carcinomas. We conclude that (1) some atypical hyperplasias demonstrate abnormal DNA content consistent with neoplastic transformation, (2) aggressive forms of intraductal carcinoma are more frequently associated with DNA content abnormalities, and (3) frequent DNA stemline heterogeneity in intraductal carcinoma supports the hypothesis that multiple genetic events occur in the development of mammary intraepithelial neoplasia.",
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