IL-8 as a urinary biomarker for the detection of bladder cancer

Virginia Urquidi, Myron Chang, Yunfeng Dai, Jeongsoon Kim, Edward D. Wolfson, Steve Goodison, Charles J. Rosser

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Background: Current urine-based assays for bladder cancer (BCa) diagnosis lack accuracy, so the search for improved biomarkers continues. Through genomic and proteomic profiling of urine, we have identified a panel of biomarkers associated with the presence of BCa. In this study, we evaluated the utility of three of these biomarkers, interleukin 8 (IL-8), Matrix metallopeptidase 9 (MMP-9) and Syndecan in the diagnosis of BCa through urinalysis. Methods. Voided urines from 127 subjects, cancer subjects (n=64), non-cancer subjects (n=63) were analyzed. The protein concentrations of IL-8, MMP-9, and Syndecan were assessed by enzyme-linked immunosorbent assay (ELISA). Data were also compared to a commercial ELISA-based BCa detection assay (BTA-Trak) and urinary cytology. We used the area under the curve of a receiver operating characteristic (AUROC) to compare the performance of each biomarker. Results: Urinary protein concentrations of IL-8, MMP-9 and BTA were significantly elevated in BCa subjects. Of the experimental markers compared to BTA-Trak, IL-8 was the most prominent marker (AUC; 0.79; 95% confidence interval [CI], 0.72-0.86). Multivariate regression analysis revealed that only IL-8 (OR; 1.51; 95% CI, 1.16-1.97, p=0.002) was an independent factor for the detection of BCa. Conclusions: These results suggest that the measurement of IL-8 in voided urinary samples may have utility for urine-based detection of BCa. These findings need to be confirmed in a larger, prospective cohort.

Original languageEnglish (US)
Article number12
JournalBMC Urology
Volume12
DOIs
StatePublished - 2012

Keywords

  • Biomarkers
  • Bladder cancer
  • Diagnosis
  • IL-8

ASJC Scopus subject areas

  • Reproductive Medicine
  • Urology

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    Urquidi, V., Chang, M., Dai, Y., Kim, J., Wolfson, E. D., Goodison, S., & Rosser, C. J. (2012). IL-8 as a urinary biomarker for the detection of bladder cancer. BMC Urology, 12, [12]. https://doi.org/10.1186/1471-2490-12-12