IL-4 regulates c-kit proto-oncogene product expression in human mast and myeloid progenitor cells

Christian Sillaber, Herbert Strobl, Dorian Bevec, Leonie K. Ashman, Joseph H. Butterfield, Klaus Lechner, Dieter Maurer, Peter Bettelheim, Peter Valent

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

The c-kit proto-oncogene encodes the receptor for a novel hemopoietic cytokine, termed stem cell factor (SCF) or mast cell growth factor (MGF) according to its stimulating spectrum. The human receptor for SCF/MGF is expressed in a subset of normal bone marrow progenitor cells, in leukemic myeloid cells, and in mast cells. In the present study, the effects of recombinant human growth regulators (IL-1 through -9, granulocyte-macrophage/granulocyte/ macrophage-CSF, IFN, and TNF) on c-kit proto-oncogene product expression were analyzed by indirect immunofluorescence, by using the anti-SCF/ MGFR mAb YB5.B8, and Northern blot analyses, by using a c-kit oligonucleotide probe. Of all cytokines tested, IL-4 was found to down-regulate expression of YB5.B8 Ag in the human mast cell line HMC-1 (maximum inhibition, 51.05 ± 16.36% mean fluorescence intensity of control; p < 0.02), as well as in primary leukemic myeloid cells. IL-4 was also found to down-regulate expression of YB5.B8 Ag in normal enriched bone marrow progenitor cells. The effects of IL-4 on expression of YB5.B8 Ag in myeloid/mast cell progenitors was dose and time dependent (maximum effects observed on days 2 and/or 4, by using 50 U/ml of rIL-4) and could be neutralized by using anti-IL-4 mAb. Moreover, IL-4 was found to down-regulate expression of c-kit mRNA in leukemic myeloid cells as well as in HMC-1 cells. Together, these observations identify IL-4 as a regulator of c-kit proto-oncogene product expression in the human system. The effects of IL-4 on human hemopoietic progenitor cells and mast cells may be mediated in part through regulation of SCF/MGFR expression.

Original languageEnglish (US)
Pages (from-to)4224-4228
Number of pages5
JournalJournal of Immunology
Volume147
Issue number12
StatePublished - Dec 15 1991

Fingerprint

Myeloid Progenitor Cells
Proto-Oncogenes
Oncogene Proteins
Interleukin-4
Stem Cell Factor
Myeloid Cells
Mast Cells
Stem Cells
Down-Regulation
Granulocytes
Bone Marrow Cells
Macrophages
Proto-Oncogene Proteins c-kit
Cytokines
Oligonucleotide Probes
Indirect Fluorescent Antibody Technique
Interleukin-1
Northern Blotting
Fluorescence
Cell Line

ASJC Scopus subject areas

  • Immunology

Cite this

Sillaber, C., Strobl, H., Bevec, D., Ashman, L. K., Butterfield, J. H., Lechner, K., ... Valent, P. (1991). IL-4 regulates c-kit proto-oncogene product expression in human mast and myeloid progenitor cells. Journal of Immunology, 147(12), 4224-4228.

IL-4 regulates c-kit proto-oncogene product expression in human mast and myeloid progenitor cells. / Sillaber, Christian; Strobl, Herbert; Bevec, Dorian; Ashman, Leonie K.; Butterfield, Joseph H.; Lechner, Klaus; Maurer, Dieter; Bettelheim, Peter; Valent, Peter.

In: Journal of Immunology, Vol. 147, No. 12, 15.12.1991, p. 4224-4228.

Research output: Contribution to journalArticle

Sillaber, C, Strobl, H, Bevec, D, Ashman, LK, Butterfield, JH, Lechner, K, Maurer, D, Bettelheim, P & Valent, P 1991, 'IL-4 regulates c-kit proto-oncogene product expression in human mast and myeloid progenitor cells', Journal of Immunology, vol. 147, no. 12, pp. 4224-4228.
Sillaber C, Strobl H, Bevec D, Ashman LK, Butterfield JH, Lechner K et al. IL-4 regulates c-kit proto-oncogene product expression in human mast and myeloid progenitor cells. Journal of Immunology. 1991 Dec 15;147(12):4224-4228.
Sillaber, Christian ; Strobl, Herbert ; Bevec, Dorian ; Ashman, Leonie K. ; Butterfield, Joseph H. ; Lechner, Klaus ; Maurer, Dieter ; Bettelheim, Peter ; Valent, Peter. / IL-4 regulates c-kit proto-oncogene product expression in human mast and myeloid progenitor cells. In: Journal of Immunology. 1991 ; Vol. 147, No. 12. pp. 4224-4228.
@article{1f4207058e344251994b2255a42c7f29,
title = "IL-4 regulates c-kit proto-oncogene product expression in human mast and myeloid progenitor cells",
abstract = "The c-kit proto-oncogene encodes the receptor for a novel hemopoietic cytokine, termed stem cell factor (SCF) or mast cell growth factor (MGF) according to its stimulating spectrum. The human receptor for SCF/MGF is expressed in a subset of normal bone marrow progenitor cells, in leukemic myeloid cells, and in mast cells. In the present study, the effects of recombinant human growth regulators (IL-1 through -9, granulocyte-macrophage/granulocyte/ macrophage-CSF, IFN, and TNF) on c-kit proto-oncogene product expression were analyzed by indirect immunofluorescence, by using the anti-SCF/ MGFR mAb YB5.B8, and Northern blot analyses, by using a c-kit oligonucleotide probe. Of all cytokines tested, IL-4 was found to down-regulate expression of YB5.B8 Ag in the human mast cell line HMC-1 (maximum inhibition, 51.05 ± 16.36{\%} mean fluorescence intensity of control; p < 0.02), as well as in primary leukemic myeloid cells. IL-4 was also found to down-regulate expression of YB5.B8 Ag in normal enriched bone marrow progenitor cells. The effects of IL-4 on expression of YB5.B8 Ag in myeloid/mast cell progenitors was dose and time dependent (maximum effects observed on days 2 and/or 4, by using 50 U/ml of rIL-4) and could be neutralized by using anti-IL-4 mAb. Moreover, IL-4 was found to down-regulate expression of c-kit mRNA in leukemic myeloid cells as well as in HMC-1 cells. Together, these observations identify IL-4 as a regulator of c-kit proto-oncogene product expression in the human system. The effects of IL-4 on human hemopoietic progenitor cells and mast cells may be mediated in part through regulation of SCF/MGFR expression.",
author = "Christian Sillaber and Herbert Strobl and Dorian Bevec and Ashman, {Leonie K.} and Butterfield, {Joseph H.} and Klaus Lechner and Dieter Maurer and Peter Bettelheim and Peter Valent",
year = "1991",
month = "12",
day = "15",
language = "English (US)",
volume = "147",
pages = "4224--4228",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "12",

}

TY - JOUR

T1 - IL-4 regulates c-kit proto-oncogene product expression in human mast and myeloid progenitor cells

AU - Sillaber, Christian

AU - Strobl, Herbert

AU - Bevec, Dorian

AU - Ashman, Leonie K.

AU - Butterfield, Joseph H.

AU - Lechner, Klaus

AU - Maurer, Dieter

AU - Bettelheim, Peter

AU - Valent, Peter

PY - 1991/12/15

Y1 - 1991/12/15

N2 - The c-kit proto-oncogene encodes the receptor for a novel hemopoietic cytokine, termed stem cell factor (SCF) or mast cell growth factor (MGF) according to its stimulating spectrum. The human receptor for SCF/MGF is expressed in a subset of normal bone marrow progenitor cells, in leukemic myeloid cells, and in mast cells. In the present study, the effects of recombinant human growth regulators (IL-1 through -9, granulocyte-macrophage/granulocyte/ macrophage-CSF, IFN, and TNF) on c-kit proto-oncogene product expression were analyzed by indirect immunofluorescence, by using the anti-SCF/ MGFR mAb YB5.B8, and Northern blot analyses, by using a c-kit oligonucleotide probe. Of all cytokines tested, IL-4 was found to down-regulate expression of YB5.B8 Ag in the human mast cell line HMC-1 (maximum inhibition, 51.05 ± 16.36% mean fluorescence intensity of control; p < 0.02), as well as in primary leukemic myeloid cells. IL-4 was also found to down-regulate expression of YB5.B8 Ag in normal enriched bone marrow progenitor cells. The effects of IL-4 on expression of YB5.B8 Ag in myeloid/mast cell progenitors was dose and time dependent (maximum effects observed on days 2 and/or 4, by using 50 U/ml of rIL-4) and could be neutralized by using anti-IL-4 mAb. Moreover, IL-4 was found to down-regulate expression of c-kit mRNA in leukemic myeloid cells as well as in HMC-1 cells. Together, these observations identify IL-4 as a regulator of c-kit proto-oncogene product expression in the human system. The effects of IL-4 on human hemopoietic progenitor cells and mast cells may be mediated in part through regulation of SCF/MGFR expression.

AB - The c-kit proto-oncogene encodes the receptor for a novel hemopoietic cytokine, termed stem cell factor (SCF) or mast cell growth factor (MGF) according to its stimulating spectrum. The human receptor for SCF/MGF is expressed in a subset of normal bone marrow progenitor cells, in leukemic myeloid cells, and in mast cells. In the present study, the effects of recombinant human growth regulators (IL-1 through -9, granulocyte-macrophage/granulocyte/ macrophage-CSF, IFN, and TNF) on c-kit proto-oncogene product expression were analyzed by indirect immunofluorescence, by using the anti-SCF/ MGFR mAb YB5.B8, and Northern blot analyses, by using a c-kit oligonucleotide probe. Of all cytokines tested, IL-4 was found to down-regulate expression of YB5.B8 Ag in the human mast cell line HMC-1 (maximum inhibition, 51.05 ± 16.36% mean fluorescence intensity of control; p < 0.02), as well as in primary leukemic myeloid cells. IL-4 was also found to down-regulate expression of YB5.B8 Ag in normal enriched bone marrow progenitor cells. The effects of IL-4 on expression of YB5.B8 Ag in myeloid/mast cell progenitors was dose and time dependent (maximum effects observed on days 2 and/or 4, by using 50 U/ml of rIL-4) and could be neutralized by using anti-IL-4 mAb. Moreover, IL-4 was found to down-regulate expression of c-kit mRNA in leukemic myeloid cells as well as in HMC-1 cells. Together, these observations identify IL-4 as a regulator of c-kit proto-oncogene product expression in the human system. The effects of IL-4 on human hemopoietic progenitor cells and mast cells may be mediated in part through regulation of SCF/MGFR expression.

UR - http://www.scopus.com/inward/record.url?scp=0026355821&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026355821&partnerID=8YFLogxK

M3 - Article

C2 - 1721642

AN - SCOPUS:0026355821

VL - 147

SP - 4224

EP - 4228

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 12

ER -