IL-4 enhances IL-3 and IL-8 gene expression in a human leukemic mast cell line

M. G. Buckley, C. M M Williams, J. Thompson, P. Pryor, K. Ray, J. H. Butterfield, J. W. Coleman

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Abstract

We examined the capacity of interleukin (IL)-4 to induce or enhance the expression of certain cytokines in resting and activated cells of the HMC-1 human leukemic mast cell line. The HMC-1 mast cells were cultured with or without recombinant human IL-4 and then activated with the calcium ionophore ionomycin. Stimulation of non-IL-4-treated cells with ionomycin (10 μM) for periods of 30 min to 8 hr induced expression of mRNA encoding IL-3, IL-4 and IL-8 but was without effect on levels of mRNA for tumour necrosis factor (TNF)-α or β-actin. Culture of the cells with IL-4 (100 ng/ml) for 24 hr led to a small increase in resting levels of mRNA for IL-3 and IL-8 but not for IL-4, TNF-α or p-actin. More notably, the IL-4 treatment produced a pronounced elevation of mRNA for IL-3 and IL-8 when the cells were subsequently activated with ionomycin. The IL-4 treatment produced a negligible effect on IL-4 mRNA, and no effect on TNF-α or p-actin mPNA levels in ionomycin-activated cells. Quantitation of cDNA by competitive polymerase chain reaction (PCR) revealed that the IL-4 treatment produced a sixfold increase in ionomycin-induced levels of cellular IL-3 mRNA, a fourfold increase in induced IL-8 mRNA and less than a twofold increase in induced IL-4 mRNA. The IL-4 treatment led to a 15- to 20-fold increase in ionomycin-induced secretion of IL-3 product and a doubling of induced IL-8 product. These effects of IL-4 were not associated with increased mast cell numbers. We conclude that IL-4 alone is a weak activator of IL-3 and IL-8 gene expression in mast cells, but is able to enhance activation signals in stimulated mast cells leading to transcription and secretion of these two cytokines.

Original languageEnglish (US)
Pages (from-to)410-415
Number of pages6
JournalImmunology
Volume84
Issue number3
StatePublished - 1995
Externally publishedYes

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Interleukin-3
Interleukin-8
Mast Cells
Interleukin-4
Gene Expression
Cell Line
Ionomycin
Messenger RNA
Actins
Tumor Necrosis Factor-alpha
Cytokines
Calcium Ionophores
Complementary DNA
Cell Culture Techniques
Cell Count

ASJC Scopus subject areas

  • Immunology

Cite this

Buckley, M. G., Williams, C. M. M., Thompson, J., Pryor, P., Ray, K., Butterfield, J. H., & Coleman, J. W. (1995). IL-4 enhances IL-3 and IL-8 gene expression in a human leukemic mast cell line. Immunology, 84(3), 410-415.

IL-4 enhances IL-3 and IL-8 gene expression in a human leukemic mast cell line. / Buckley, M. G.; Williams, C. M M; Thompson, J.; Pryor, P.; Ray, K.; Butterfield, J. H.; Coleman, J. W.

In: Immunology, Vol. 84, No. 3, 1995, p. 410-415.

Research output: Contribution to journalArticle

Buckley, MG, Williams, CMM, Thompson, J, Pryor, P, Ray, K, Butterfield, JH & Coleman, JW 1995, 'IL-4 enhances IL-3 and IL-8 gene expression in a human leukemic mast cell line', Immunology, vol. 84, no. 3, pp. 410-415.
Buckley MG, Williams CMM, Thompson J, Pryor P, Ray K, Butterfield JH et al. IL-4 enhances IL-3 and IL-8 gene expression in a human leukemic mast cell line. Immunology. 1995;84(3):410-415.
Buckley, M. G. ; Williams, C. M M ; Thompson, J. ; Pryor, P. ; Ray, K. ; Butterfield, J. H. ; Coleman, J. W. / IL-4 enhances IL-3 and IL-8 gene expression in a human leukemic mast cell line. In: Immunology. 1995 ; Vol. 84, No. 3. pp. 410-415.
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AU - Butterfield, J. H.

AU - Coleman, J. W.

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