IgH translocations in multiple myeloma: A nearly universal event that rarely involves c-myc

Peter Leif Bergsagel, E. Nardini, L. Brents, Marta Chesi, W. M. Kuehl

Research output: Contribution to journalArticle

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Abstract

Dysregulation of c-myc by translocation to the switch regions of the IgH locus occurs in most murine plasmacytomas. Translocations involving 14q32 have been reported in 20-40% of abnormal karyotypes from human multiple myeloma (MM), and involve a variety of loci. Using cytogenetics, FISH and a Southern blot assay, we analyzed 21 MM cell lines and one plasma cell leukemia and identified evidence of a 14q32 translocation in 20/22 samples. The partner loci involved are 11q13 in 6 (associated with cyclin D1 expression), 4p16 in 6 (associated with FGFR3 expression), unidentified in 3 and 1p13, 6, gq24, 12q24, 16q23, and 21q22 once each. We conclude that conventional karyotypes underestimate the frequency of 14q32 translocations in MM, where they appear to be a nearly universal event. The translocations most frequently involve IgH switch regions, and include two recurrent partner loci (11q13 and 4p16) and a promiscuous array of other partner loci. Although c-myc appears to be cis-dysregulated frequently in MM, it is only rarely translocated to the IgH locus.

Original languageEnglish (US)
Pages (from-to)283-287
Number of pages5
JournalCurrent Topics in Microbiology and Immunology
Volume224
StatePublished - 1997
Externally publishedYes

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Multiple Myeloma
Plasma Cell Leukemia
Abnormal Karyotype
Plasmacytoma
Cyclin D1
Southern Blotting
Karyotype
Cytogenetics
Cell Line

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology (medical)
  • Immunology and Microbiology(all)
  • Microbiology

Cite this

IgH translocations in multiple myeloma : A nearly universal event that rarely involves c-myc. / Bergsagel, Peter Leif; Nardini, E.; Brents, L.; Chesi, Marta; Kuehl, W. M.

In: Current Topics in Microbiology and Immunology, Vol. 224, 1997, p. 283-287.

Research output: Contribution to journalArticle

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AU - Kuehl, W. M.

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