Identification of very early lymphoid precursors in bone marrow and their regulation by estrogen

K. L. Medina; K. P. Garrett; L. F. Thompson; M. I.D. Rossi; K. J. Payne; P. W. Kincade

doi:10.1038/90659

Identification of very early lymphoid precursors in bone marrow and their regulation by estrogen

K. L. Medina, K. P. Garrett, L. F. Thompson, M. I.D. Rossi, K. J. Payne, P. W. Kincade

Immunology

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187 Scopus citations

Abstract

Estrogen is a negative regulator of lymphopoiesis and provides an experimental tool for probing relationships between lymphocyte precursors and stem cells. We found that expression of lymphocyte-associated genes and immunoglobulin (Ig) gene rearrangement occurred before CD45R acquisition. Lymphoid-restricted progenitors that were Lin^-IL-7Rα⁺c-kit^loTdT⁺ (lineage marker^-, interleukin receptor 7α⁺, c-kit^lo and terminal deoxynucleotidyl transferase⁺) were selectively depleted in estrogen-treated mice; within a less differentiated Lin^-c-kit^hi fraction, functional precursors of B and T, but not myeloid, cells were also selectively depleted. TdT and an Ig heavy chain transgene were detected within a hormone-regulated Lin^-c-kit^hiSca-I ⁺CD27⁺Flk-2⁺IL-7Rα^- subset of this multipotential progenitor population. Identification of these extremely early lymphoid precursors should facilitate investigation of the molecular mechanisms that control lineage-fate decisions in hematopoiesis.

Original language	English (US)
Pages (from-to)	718-724
Number of pages	7
Journal	Nature immunology
Volume	2
Issue number	8
DOIs	https://doi.org/10.1038/90659
State	Published - 2001

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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title = "Identification of very early lymphoid precursors in bone marrow and their regulation by estrogen",

abstract = "Estrogen is a negative regulator of lymphopoiesis and provides an experimental tool for probing relationships between lymphocyte precursors and stem cells. We found that expression of lymphocyte-associated genes and immunoglobulin (Ig) gene rearrangement occurred before CD45R acquisition. Lymphoid-restricted progenitors that were Lin-IL-7Rα+c-kitloTdT+ (lineage marker-, interleukin receptor 7α+, c-kitlo and terminal deoxynucleotidyl transferase+) were selectively depleted in estrogen-treated mice; within a less differentiated Lin-c-kithi fraction, functional precursors of B and T, but not myeloid, cells were also selectively depleted. TdT and an Ig heavy chain transgene were detected within a hormone-regulated Lin-c-kithiSca-I +CD27+Flk-2+IL-7Rα- subset of this multipotential progenitor population. Identification of these extremely early lymphoid precursors should facilitate investigation of the molecular mechanisms that control lineage-fate decisions in hematopoiesis.",

author = "Medina, {K. L.} and Garrett, {K. P.} and Thompson, {L. F.} and Rossi, {M. I.D.} and Payne, {K. J.} and Kincade, {P. W.}",

note = "Funding Information: Acknowledgements We thank L. Borghesi for critical review of the manuscript and V. Dandapani for flow cytometry and sorting expertise. Supported by the National Institutes of Health (AI 20069).",

year = "2001",

doi = "10.1038/90659",

language = "English (US)",

volume = "2",

pages = "718--724",

journal = "Nature immunology",

issn = "1529-2908",

publisher = "Nature Publishing Group",

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T1 - Identification of very early lymphoid precursors in bone marrow and their regulation by estrogen

AU - Medina, K. L.

AU - Garrett, K. P.

AU - Thompson, L. F.

AU - Rossi, M. I.D.

AU - Payne, K. J.

AU - Kincade, P. W.

N1 - Funding Information: Acknowledgements We thank L. Borghesi for critical review of the manuscript and V. Dandapani for flow cytometry and sorting expertise. Supported by the National Institutes of Health (AI 20069).

PY - 2001

Y1 - 2001

N2 - Estrogen is a negative regulator of lymphopoiesis and provides an experimental tool for probing relationships between lymphocyte precursors and stem cells. We found that expression of lymphocyte-associated genes and immunoglobulin (Ig) gene rearrangement occurred before CD45R acquisition. Lymphoid-restricted progenitors that were Lin-IL-7Rα+c-kitloTdT+ (lineage marker-, interleukin receptor 7α+, c-kitlo and terminal deoxynucleotidyl transferase+) were selectively depleted in estrogen-treated mice; within a less differentiated Lin-c-kithi fraction, functional precursors of B and T, but not myeloid, cells were also selectively depleted. TdT and an Ig heavy chain transgene were detected within a hormone-regulated Lin-c-kithiSca-I +CD27+Flk-2+IL-7Rα- subset of this multipotential progenitor population. Identification of these extremely early lymphoid precursors should facilitate investigation of the molecular mechanisms that control lineage-fate decisions in hematopoiesis.

AB - Estrogen is a negative regulator of lymphopoiesis and provides an experimental tool for probing relationships between lymphocyte precursors and stem cells. We found that expression of lymphocyte-associated genes and immunoglobulin (Ig) gene rearrangement occurred before CD45R acquisition. Lymphoid-restricted progenitors that were Lin-IL-7Rα+c-kitloTdT+ (lineage marker-, interleukin receptor 7α+, c-kitlo and terminal deoxynucleotidyl transferase+) were selectively depleted in estrogen-treated mice; within a less differentiated Lin-c-kithi fraction, functional precursors of B and T, but not myeloid, cells were also selectively depleted. TdT and an Ig heavy chain transgene were detected within a hormone-regulated Lin-c-kithiSca-I +CD27+Flk-2+IL-7Rα- subset of this multipotential progenitor population. Identification of these extremely early lymphoid precursors should facilitate investigation of the molecular mechanisms that control lineage-fate decisions in hematopoiesis.

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Identification of very early lymphoid precursors in bone marrow and their regulation by estrogen

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