Identification of two related markers for common acute lymphoblastic leukemia as heat shock proteins

John R. Strahler, Rork Kuick, Christoph Eckerskorn, Friedrich Lottspeich, Bruce C. Richardson, David A. Fox, Lloyd M. Stoolman, Curtis A. Hanson, Dean Nichols, H. Jane Tueche, Samir M. Hanash

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

By direct analysis of the polypeptide constituents of leukemic cells, we have previously detected several polypeptides that are restricted in their expression to acute lymphoblastic leukemia (ALL). In this study, we provide evidence that two polypeptides designated L2 and L4 are structurally related and represent novel markers for common ALL. Partial amino acid sequence analysis did not uncover differences between L2 and L4. The sequences obtained correspond to a previously cloned human gene designated hsp27 that is expressed, following heat shock treatment, in a variety of cells. 32Pi incorporation studies indicate that L4 is an unphosphorylated form and L2 is a phosphorylated form of hsp27. The two forms were inducible by heat shock in leukemic and nonleukemic lymphoid cells. Thus, in acute leukemia, the common ALL subtype is uniquely characterized by the constitutive expression of a polypeptide that represents a major cellular phosphoprotein.

Original languageEnglish (US)
Pages (from-to)200-207
Number of pages8
JournalJournal of Clinical Investigation
Volume85
Issue number1
StatePublished - Jan 1990
Externally publishedYes

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Keywords

  • Acute lymphoblastic leukemia
  • Heat shock protein
  • Polypeptide marker

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Strahler, J. R., Kuick, R., Eckerskorn, C., Lottspeich, F., Richardson, B. C., Fox, D. A., Stoolman, L. M., Hanson, C. A., Nichols, D., Tueche, H. J., & Hanash, S. M. (1990). Identification of two related markers for common acute lymphoblastic leukemia as heat shock proteins. Journal of Clinical Investigation, 85(1), 200-207.