Identification of three new alternate human kallikrein 2 transcripts

Evidence of long transcript and alternative splicing

Xiu Fen Liu, Magnus Essand, George Vasmatzis, Byungkook Lee, Ira Pastan

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

In a search for prostate specific genes in the human expressed sequence tag (EST) database, we identified a seemingly unique EST cluster C81. Experimental data linked C81 to the human hKLK2 gene that encodes a prostate specific serine protease-human glandular kallikrein (hK2). We uncovered a full-length hKLK2 cDNA corresponding to a 3.0 kb hKLK2 mRNA by PCR and sequence analysis. The 3.0 kb transcript accounts for about 25% of the hKLK2 transcripts as compared to the previously known 1.5 k.b transcript. We also identified a third spliced form of the hKLK2 gene produced by alternative splicing between intron III and exon 4. This spliced form was detected in normal prostate, prostate cancer and the prostate adenocarcinoma cell line LNCaP. The identification of long hKLK2 transcript and an alternative spliced form of the hKLK2 gene indicates that regulation of the gene is complex.

Original languageEnglish (US)
Pages (from-to)833-839
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume264
Issue number3
DOIs
StatePublished - Nov 2 1999
Externally publishedYes

Fingerprint

Tissue Kallikreins
Alternative Splicing
Genes
Prostate
Expressed Sequence Tags
Prostatic Neoplasms
Serine Proteases
Introns
Sequence Analysis
Exons
Adenocarcinoma
Complementary DNA
Cells
Databases
Cell Line
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Alternative splicing
  • EST
  • hKLK2 gene
  • Kallikrein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of three new alternate human kallikrein 2 transcripts : Evidence of long transcript and alternative splicing. / Liu, Xiu Fen; Essand, Magnus; Vasmatzis, George; Lee, Byungkook; Pastan, Ira.

In: Biochemical and Biophysical Research Communications, Vol. 264, No. 3, 02.11.1999, p. 833-839.

Research output: Contribution to journalArticle

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