Identification of TDE2 gene and its expression in non-small cell lung cancer

Audrey Player, John Gillespie, Takeshi Fujii, Junya Fukuoka, Tatiana Dracheva, Dauod Meerzaman, Kyeong Man Hong, John Curran, Goziam Attoh, William Travis, Jin Jen

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17 Scopus citations

Abstract

TDE2, a gene with sequence similarity to the mouse testicular tumor-differentially-expressed (Tde1/MUSTETU) gene, was identified by serial analysis of gene expression (SAGE) in non-small cell lung cancers (NSCLC). Here we characterized the TDE2 gene and determined its transcript levels in a panel of lung tumors, adjacent nonmalignant lung tissues and a variety of normal human tissues. In addition, we show that TDE2 is a potential transmembrane protein with 11 putative transmembrane helices. Using real-time quantitative PCR, we showed that TDE2 transcript levels were higher in NSCLC compared to nonmalignant samples. In nonpulmonary normal tissues, the level of TDE2 was the highest in bladder, kidney and muscle; moderate to low in stomach, liver, skin, placenta and ovary tissues; and undetectable in brain, spleen and heart. By in situ hybridization, we showed that 10 of 18 lung tumors and only 1 of 14 adjacent nonmalignant regions had high levels of TDE2 transcripts. Alternatively, only 2 of 18 tumors and 8 of 14 adjacent nonmalignant bronchiole epithelium regions demonstrated negative to low levels of TDE2 signals.

Original languageEnglish (US)
Pages (from-to)238-243
Number of pages6
JournalInternational Journal of Cancer
Volume107
Issue number2
DOIs
StatePublished - Nov 1 2003

Keywords

  • In situ hybridization
  • Non-small cell lung cancer
  • Serial analysis of gene expression (SAGE)
  • TDE1
  • TDE2
  • Tms1
  • Tms2

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Player, A., Gillespie, J., Fujii, T., Fukuoka, J., Dracheva, T., Meerzaman, D., Hong, K. M., Curran, J., Attoh, G., Travis, W., & Jen, J. (2003). Identification of TDE2 gene and its expression in non-small cell lung cancer. International Journal of Cancer, 107(2), 238-243. https://doi.org/10.1002/ijc.11391