Identification of T-cell receptor Vβ deletion mutant mouse strain AU/ssJ (H-2q) which is resistant to collagen-induced arthritis

Tariq M. Haqqi, Subhashis Banerjee, Wendy L. Jones, Gary Anderson, Mark A. Behlke, Dennis Y. Loh, Harvinder S. Luthra, Chella S. David

Research output: Contribution to journalArticle

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Abstract

Our laboratory is involved in investigating the role of T-cell receptor (Tcr) in collagen-induced arthritis (CIA). During these studies we found AU/ssJ (H-2q) mice to be resistant to CIA like SWR (H-2q), as compared with other H-2q strains with wild-type Tcr like DBA/1 and B 10. Q. Upon screening with monoclonal antibodies F23.1 and KJ23a, AU/ssJ was found to be F23.1 negative (Vβ8 Tcr negative) and KJ23a positive (Vβ17a Tcr positive). Southern blot analysis on liver DNA using specific Tcr-Vβ probes confirmed the deletion of Vβ8 gene family and also showed that AU/ssJ mice have deletions of Vβ9, Vβ13, Vβ12, and Vβ11 genes of Tcr. Further, these mice show a restriction fragment length polymorphism pattern with Vβ10, Vβ6, and Vβ17 probes similar to SWR mice as compared with 1310 mice. Since SWR and AU/ssJ are from different backgrounds, these studies indicate that specific variable region β chain genes of Tcr are crucial for susceptibility to CIA in mice. Furthermore, these studies identify an additional inbred strain which has also deleted 50% of its Tcr-Vβ genes.

Original languageEnglish (US)
Pages (from-to)180-185
Number of pages6
JournalImmunogenetics
Volume29
Issue number3
DOIs
StatePublished - Mar 1989

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Mutant Strains Mice
Experimental Arthritis
T-Cell Antigen Receptor
T-Cell Receptor Genes
Gene Deletion
Southern Blotting
Restriction Fragment Length Polymorphisms
Monoclonal Antibodies
Liver
DNA

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Haqqi, T. M., Banerjee, S., Jones, W. L., Anderson, G., Behlke, M. A., Loh, D. Y., ... David, C. S. (1989). Identification of T-cell receptor Vβ deletion mutant mouse strain AU/ssJ (H-2q) which is resistant to collagen-induced arthritis. Immunogenetics, 29(3), 180-185. https://doi.org/10.1007/BF00373643

Identification of T-cell receptor Vβ deletion mutant mouse strain AU/ssJ (H-2q) which is resistant to collagen-induced arthritis. / Haqqi, Tariq M.; Banerjee, Subhashis; Jones, Wendy L.; Anderson, Gary; Behlke, Mark A.; Loh, Dennis Y.; Luthra, Harvinder S.; David, Chella S.

In: Immunogenetics, Vol. 29, No. 3, 03.1989, p. 180-185.

Research output: Contribution to journalArticle

Haqqi, TM, Banerjee, S, Jones, WL, Anderson, G, Behlke, MA, Loh, DY, Luthra, HS & David, CS 1989, 'Identification of T-cell receptor Vβ deletion mutant mouse strain AU/ssJ (H-2q) which is resistant to collagen-induced arthritis', Immunogenetics, vol. 29, no. 3, pp. 180-185. https://doi.org/10.1007/BF00373643
Haqqi, Tariq M. ; Banerjee, Subhashis ; Jones, Wendy L. ; Anderson, Gary ; Behlke, Mark A. ; Loh, Dennis Y. ; Luthra, Harvinder S. ; David, Chella S. / Identification of T-cell receptor Vβ deletion mutant mouse strain AU/ssJ (H-2q) which is resistant to collagen-induced arthritis. In: Immunogenetics. 1989 ; Vol. 29, No. 3. pp. 180-185.
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