Abstract
A new isotope-assisted cross-relaxation editing experiment, [1H-13C]DINE-NOESY[1H-15N]HSQC (DINE = Double INEPT Edited), is proposed. It is based on the selective inversion of CH/CH3, or CH2 protons in the middle of the mixing time. The experiment sorts out the spin diffusion paths according to the principal mediators, either the CH/CH3 or the CH2 protons. This is useful in the structure refinement process, as it enables proper alignment of the aliphatic protons in the vicinity of NH protons.
Original language | English (US) |
---|---|
Pages (from-to) | 317-322 |
Number of pages | 6 |
Journal | Journal of Biomolecular NMR |
Volume | 9 |
Issue number | 3 |
DOIs | |
State | Published - 1997 |
Keywords
- NOESY editing
- Protein NMR
- Spin diffusion
ASJC Scopus subject areas
- Biochemistry
- Spectroscopy