Identification of spin diffusion pathways in proteins by isotope-assisted NMR cross-relaxation network editing

Nenad Juranić, Zsolt Zolnai, Slobodan Macura

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

A new isotope-assisted cross-relaxation editing experiment, [1H-13C]DINE-NOESY[1H-15N]HSQC (DINE = Double INEPT Edited), is proposed. It is based on the selective inversion of CH/CH3, or CH2 protons in the middle of the mixing time. The experiment sorts out the spin diffusion paths according to the principal mediators, either the CH/CH3 or the CH2 protons. This is useful in the structure refinement process, as it enables proper alignment of the aliphatic protons in the vicinity of NH protons.

Original languageEnglish (US)
Pages (from-to)317-322
Number of pages6
JournalJournal of Biomolecular NMR
Volume9
Issue number3
DOIs
StatePublished - Jan 1 1997

Keywords

  • NOESY editing
  • Protein NMR
  • Spin diffusion

ASJC Scopus subject areas

  • Biochemistry
  • Spectroscopy

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