Identification of novel rodent-borne orthohantaviruses in an endemic area of chronic kidney disease of unknown etiology (Ckdu) in Sri Lanka

Devinda S. Muthusinghe; Kenta Shimizu; Sithumini M.W. Lokupathirage; Zhouoxing Wei; Yomani D. Sarathkumara; G. R. Amanda Fonseka; Pavani Senarathne; Nobuo Koizumi; Tomonori Kawakami; Akio Koizumi; Chaminda Wickramasinghe; Hideki Ebihara; Keita Matsuno; Yoshimi Tsuda; Jiro Arikawa; Chandika D. Gamage; Kumiko Yoshimatsu

doi:10.3390/v13101984

Identification of novel rodent-borne orthohantaviruses in an endemic area of chronic kidney disease of unknown etiology (Ckdu) in Sri Lanka

Devinda S. Muthusinghe, Kenta Shimizu, Sithumini M.W. Lokupathirage, Zhouoxing Wei, Yomani D. Sarathkumara, G. R. Amanda Fonseka, Pavani Senarathne, Nobuo Koizumi, Tomonori Kawakami, Akio Koizumi, Chaminda Wickramasinghe, Hideki Ebihara, Keita Matsuno, Yoshimi Tsuda, Jiro Arikawa, Chandika D. Gamage, Kumiko Yoshimatsu

Molecular Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

We reported the genetic evidence of circulating hantaviruses from small mammals captured in a chronic kidney disease of unknown etiology (CKDu) hotspot area of Sri Lanka. The high seroprevalence of anti-hantavirus antibodies against Thailand orthohantavirus (THAIV) has been reported among CKDu patients and rodents in Sri Lankan CKDu hotspots. We captured 116 small mammals from CKDu endemic regions in the Polonnaruwa District of Sri Lanka. Seven animals (five out of 11 Mus booduga and two out of 99 Rattus rattus) were PCR-positive for the hantavirus. A rat-borne sequence was grouped with a THAIV-like Anjozorobe virus. In contrast, Mus-borne sequences belonged to the THAIV lineage, suggesting a novel orthohantavirus species according to the phylogenetic analyses and whole-genome comparisons. Our genetic evidence indicates the presence of two THAIV-related viruses circulating in this CKDu endemic area, suggesting a basis for further investigations to identify the infectious virus in patients with CKDu and the CKDu induction mechanism of these viruses.

Original language	English (US)
Article number	1984
Journal	Viruses
Volume	13
Issue number	10
DOIs	https://doi.org/10.3390/v13101984
State	Published - Oct 2021

Keywords

Anjozorobe hantavirus
Hantavirus
Mus booduga
Thailand orthohantavirus

ASJC Scopus subject areas

Infectious Diseases
Virology

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Muthusinghe, D. S., Shimizu, K., Lokupathirage, S. M. W., Wei, Z., Sarathkumara, Y. D., Amanda Fonseka, G. R., Senarathne, P., Koizumi, N., Kawakami, T., Koizumi, A., Wickramasinghe, C., Ebihara, H., Matsuno, K., Tsuda, Y., Arikawa, J., Gamage, C. D., & Yoshimatsu, K. (2021). Identification of novel rodent-borne orthohantaviruses in an endemic area of chronic kidney disease of unknown etiology (Ckdu) in Sri Lanka. Viruses, 13(10), Article 1984. https://doi.org/10.3390/v13101984

Muthusinghe, DS, Shimizu, K, Lokupathirage, SMW, Wei, Z, Sarathkumara, YD, Amanda Fonseka, GR, Senarathne, P, Koizumi, N, Kawakami, T, Koizumi, A, Wickramasinghe, C, Ebihara, H, Matsuno, K, Tsuda, Y, Arikawa, J, Gamage, CD & Yoshimatsu, K 2021, 'Identification of novel rodent-borne orthohantaviruses in an endemic area of chronic kidney disease of unknown etiology (Ckdu) in Sri Lanka', Viruses, vol. 13, no. 10, 1984. https://doi.org/10.3390/v13101984

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title = "Identification of novel rodent-borne orthohantaviruses in an endemic area of chronic kidney disease of unknown etiology (Ckdu) in Sri Lanka",

abstract = "We reported the genetic evidence of circulating hantaviruses from small mammals captured in a chronic kidney disease of unknown etiology (CKDu) hotspot area of Sri Lanka. The high seroprevalence of anti-hantavirus antibodies against Thailand orthohantavirus (THAIV) has been reported among CKDu patients and rodents in Sri Lankan CKDu hotspots. We captured 116 small mammals from CKDu endemic regions in the Polonnaruwa District of Sri Lanka. Seven animals (five out of 11 Mus booduga and two out of 99 Rattus rattus) were PCR-positive for the hantavirus. A rat-borne sequence was grouped with a THAIV-like Anjozorobe virus. In contrast, Mus-borne sequences belonged to the THAIV lineage, suggesting a novel orthohantavirus species according to the phylogenetic analyses and whole-genome comparisons. Our genetic evidence indicates the presence of two THAIV-related viruses circulating in this CKDu endemic area, suggesting a basis for further investigations to identify the infectious virus in patients with CKDu and the CKDu induction mechanism of these viruses.",

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AU - Amanda Fonseka, G. R.

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AU - Koizumi, Nobuo

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AU - Koizumi, Akio

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AU - Yoshimatsu, Kumiko

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Identification of novel rodent-borne orthohantaviruses in an endemic area of chronic kidney disease of unknown etiology (Ckdu) in Sri Lanka

Abstract

Keywords

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