Identification of novel protein biomarkers of preterm birth in human cervical-vaginal fluid

Leonardo Pereira, Ashok P. Reddy, Thomas Jacob, Archana Thomas, Kimberly A. Schneider, Surendra Dasari, Jodi A. Lapidus, Xinfang Lu, Matthew Rodland, Charles T. Roberts, Michael G. Gravett, Srinivasa R. Nagalla

Research output: Contribution to journalArticle

101 Scopus citations

Abstract

Spontaneous preterm birth (SPTB) is a major contributor to perinatal morbidity and mortality. However, the diagnosis of preterm labor (PTL) that leads to preterm birth is difficult, and there is a pressing need for improved diagnosis. We utilized multidimensional liquid chromatography-tandem mass spectrometry (LC/LC-MS/MS; MudPIT) and Fluorescence two-dimensional differential in-gel electrophoresis (2D-DIGE) to identify potential biomarkers of PTL and SPTB. MudPIT analysis identified 205 proteins in cervical-vaginal fluid (CVF), 28 of which exhibited significant differences in pairwise and progressive comparisons. Calgranulins, annexins, S100 calcium-binding protein A7, and epidermal fatty acid binding protein were abundant in CVF and differentially present in PTL and SPTB samples, as were the serum proteins α-1- antitrypsin, α1-acid glycoprotein, haptoglobin, serotransferrin, and vitamin D binding protein. 2D-DIGE identified 17 proteins that were significantly differentially present in PTL and SPTB. Immunoblotting with specific antibodies confirmed the differences and trends of selected markers. Further characterization and quantification of these markers in a larger cohort of subjects may provide the basis for new tests for the early, noninvasive positive prediction of SPTB.

Original languageEnglish (US)
Pages (from-to)1269-1276
Number of pages8
JournalJournal of Proteome Research
Volume6
Issue number4
DOIs
StatePublished - Apr 1 2007

Keywords

  • Biomarkers
  • Pregnancy
  • Prematurity
  • Preterm labor
  • Vaginal fluid

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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    Pereira, L., Reddy, A. P., Jacob, T., Thomas, A., Schneider, K. A., Dasari, S., Lapidus, J. A., Lu, X., Rodland, M., Roberts, C. T., Gravett, M. G., & Nagalla, S. R. (2007). Identification of novel protein biomarkers of preterm birth in human cervical-vaginal fluid. Journal of Proteome Research, 6(4), 1269-1276. https://doi.org/10.1021/pr0605421