Abstract
Chromogenic in situ hybridization (CISH) is a recently developed technique, which utilizes the general principles of in situ hybridization and a detection system similar to immunohistochemistry. To assess the utility of CISH for analysis of MYCN gene amplification, we compared this assay with established diagnostic assays such as Southern blot analysis (SB) and fluorescent in situ hybridization (FISH), CISH was performed on 67 cases of neuroblastoma using tissue microarray (65 cases) and whole tissue sections (2 cases), Unequivocal, high-level amplification (≥10 gene copies per tumor nucleus) was identified in 19 of 67 (28,4%) tumors, Two (3%) tumors showed low-level amplification (6-9 gene copies per tumor nucleus). No amplification was seen in 46 of 67 (68.6%) tumors. SB data were available in 44 tumors. Forty-one of the 44 tumors (93%) showed concordant results between CISH and SB. Three tumors showed MYCN amplification by CISH but no amplification by SB, most likely due to dilution effect of nonneoplastic tissue in the test samples. Two of these three tumors also showed MYCN amplification by FISH, and the third tumor was not analyzed by FISH. FISH data were available in total of 30 tumors. All 30 tumors showed concordant results between CISH and FISH for classifying a tumor as MYCN amplified or not amplified. We conclude that CISH is an accurate method for determining MYCN gene amplification, with added advantages that make it a more practically useful method.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 72-76 |
| Number of pages | 5 |
| Journal | Diagnostic Molecular Pathology |
| Volume | 14 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jun 2005 |
| Externally published | Yes |
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Keywords
- Chromogenic in situ hybridization
- CISH
- MYCN
- Neuroblastoma
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Identification of MYCN gene amplification in neuroblastoma using chromogenic in situ hybridization (CISH). / Bhargava, Rohit; Oppenheimer, Orit; Gerald, William; Jhanwar, Suresh C.; Chen, Beiyun.
In: Diagnostic Molecular Pathology, Vol. 14, No. 2, 06.2005, p. 72-76.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of MYCN gene amplification in neuroblastoma using chromogenic in situ hybridization (CISH)
AU - Bhargava, Rohit
AU - Oppenheimer, Orit
AU - Gerald, William
AU - Jhanwar, Suresh C.
AU - Chen, Beiyun
PY - 2005/6
Y1 - 2005/6
N2 - Chromogenic in situ hybridization (CISH) is a recently developed technique, which utilizes the general principles of in situ hybridization and a detection system similar to immunohistochemistry. To assess the utility of CISH for analysis of MYCN gene amplification, we compared this assay with established diagnostic assays such as Southern blot analysis (SB) and fluorescent in situ hybridization (FISH), CISH was performed on 67 cases of neuroblastoma using tissue microarray (65 cases) and whole tissue sections (2 cases), Unequivocal, high-level amplification (≥10 gene copies per tumor nucleus) was identified in 19 of 67 (28,4%) tumors, Two (3%) tumors showed low-level amplification (6-9 gene copies per tumor nucleus). No amplification was seen in 46 of 67 (68.6%) tumors. SB data were available in 44 tumors. Forty-one of the 44 tumors (93%) showed concordant results between CISH and SB. Three tumors showed MYCN amplification by CISH but no amplification by SB, most likely due to dilution effect of nonneoplastic tissue in the test samples. Two of these three tumors also showed MYCN amplification by FISH, and the third tumor was not analyzed by FISH. FISH data were available in total of 30 tumors. All 30 tumors showed concordant results between CISH and FISH for classifying a tumor as MYCN amplified or not amplified. We conclude that CISH is an accurate method for determining MYCN gene amplification, with added advantages that make it a more practically useful method.
AB - Chromogenic in situ hybridization (CISH) is a recently developed technique, which utilizes the general principles of in situ hybridization and a detection system similar to immunohistochemistry. To assess the utility of CISH for analysis of MYCN gene amplification, we compared this assay with established diagnostic assays such as Southern blot analysis (SB) and fluorescent in situ hybridization (FISH), CISH was performed on 67 cases of neuroblastoma using tissue microarray (65 cases) and whole tissue sections (2 cases), Unequivocal, high-level amplification (≥10 gene copies per tumor nucleus) was identified in 19 of 67 (28,4%) tumors, Two (3%) tumors showed low-level amplification (6-9 gene copies per tumor nucleus). No amplification was seen in 46 of 67 (68.6%) tumors. SB data were available in 44 tumors. Forty-one of the 44 tumors (93%) showed concordant results between CISH and SB. Three tumors showed MYCN amplification by CISH but no amplification by SB, most likely due to dilution effect of nonneoplastic tissue in the test samples. Two of these three tumors also showed MYCN amplification by FISH, and the third tumor was not analyzed by FISH. FISH data were available in total of 30 tumors. All 30 tumors showed concordant results between CISH and FISH for classifying a tumor as MYCN amplified or not amplified. We conclude that CISH is an accurate method for determining MYCN gene amplification, with added advantages that make it a more practically useful method.
KW - Chromogenic in situ hybridization
KW - CISH
KW - MYCN
KW - Neuroblastoma
UR - http://www.scopus.com/inward/record.url?scp=19544383792&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=19544383792&partnerID=8YFLogxK
U2 - 10.1097/01.pas.0000149878.78117.ff
DO - 10.1097/01.pas.0000149878.78117.ff
M3 - Article
VL - 14
SP - 72
EP - 76
JO - Diagnostic Molecular Pathology
JF - Diagnostic Molecular Pathology
SN - 1052-9551
IS - 2
ER -