Identification of human glandular kallikrein hK2 from LNCaP cells

Lana S. Grauer, M. Cristine Charlesworth, Mohammad S. Saedi, Judith A. Finlay, Ru Shya Liu, Kristine Kuus-Reichel, Charles Y F Young, Donald J. Tindall

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Based on studies indicating that human glandular kallikrein (hK2) mRNA is present in the prostate, we prepared a monoclonal antibody to a synthetic peptide corresponding to the 41-56 region of hK2 to try to identify the hK2 protein. Although prostate-specific antigen (PSA) and hK2 share 80% homology, the 41-56 amine acid sequence of hK2 is only 50% homologous with PSA. A monoclonal antibody, HK1A523, was identified that demonstrates high specificity for hK2. In western blot analysis, the antibody has a 1,000-fold greater sensitivity for the detection of hK2 than for PSA. The antibody was used to probe spent media from the prostate carcinoma cell line, LNCaP. An immunoreactive species was N-terminally sequenced and identified as mature hK2. HK1A523 was also utilized to probe prostate tumor cytosols and seminal fluid where putative forms of hK2 were also identified. The hK2 protein therefore is expressed and secreted from prostate carcinoma cells.

Original languageEnglish (US)
Pages (from-to)353-359
Number of pages7
JournalJournal of Andrology
Volume17
Issue number4
StatePublished - 1996
Externally publishedYes

Fingerprint

Tissue Kallikreins
Prostate
Prostate-Specific Antigen
Monoclonal Antibodies
Carcinoma
Antibodies
Cytosol
Amines
Proteins
Western Blotting
Cell Line
Messenger RNA
Peptides
Acids
Neoplasms

Keywords

  • monoclonal antibody
  • Prostate carcinoma
  • seminal fluid

ASJC Scopus subject areas

  • Endocrinology

Cite this

Grauer, L. S., Charlesworth, M. C., Saedi, M. S., Finlay, J. A., Liu, R. S., Kuus-Reichel, K., ... Tindall, D. J. (1996). Identification of human glandular kallikrein hK2 from LNCaP cells. Journal of Andrology, 17(4), 353-359.

Identification of human glandular kallikrein hK2 from LNCaP cells. / Grauer, Lana S.; Charlesworth, M. Cristine; Saedi, Mohammad S.; Finlay, Judith A.; Liu, Ru Shya; Kuus-Reichel, Kristine; Young, Charles Y F; Tindall, Donald J.

In: Journal of Andrology, Vol. 17, No. 4, 1996, p. 353-359.

Research output: Contribution to journalArticle

Grauer, LS, Charlesworth, MC, Saedi, MS, Finlay, JA, Liu, RS, Kuus-Reichel, K, Young, CYF & Tindall, DJ 1996, 'Identification of human glandular kallikrein hK2 from LNCaP cells', Journal of Andrology, vol. 17, no. 4, pp. 353-359.
Grauer LS, Charlesworth MC, Saedi MS, Finlay JA, Liu RS, Kuus-Reichel K et al. Identification of human glandular kallikrein hK2 from LNCaP cells. Journal of Andrology. 1996;17(4):353-359.
Grauer, Lana S. ; Charlesworth, M. Cristine ; Saedi, Mohammad S. ; Finlay, Judith A. ; Liu, Ru Shya ; Kuus-Reichel, Kristine ; Young, Charles Y F ; Tindall, Donald J. / Identification of human glandular kallikrein hK2 from LNCaP cells. In: Journal of Andrology. 1996 ; Vol. 17, No. 4. pp. 353-359.
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