TY - JOUR
T1 - Identification of genes associated with tumorigenesis of meibomian cell carcinoma by microarray analysis
AU - Kumar, Arun
AU - Kumar Dorairaj, Syril
AU - Prabhakaran, Venkatesh C.
AU - Prakash, D. Ravi
AU - Chakraborty, Sanjukta
N1 - Funding Information:
We are very grateful to the patients and normal individuals for taking part in this study. We also thank Mr. Aiyaz Mohamed, Mr. V. Madavan, and Dr. S. Khare for technical help. This work was financially supported by a grant from the ICMR, New Delhi. We also thank three anonymous reviewers and the associate editor, Professor Sandrine Dudoit, for their valuable suggestions to improve the manuscript.
PY - 2007/11
Y1 - 2007/11
N2 - Meibomian cell carcinoma (MCC) is a malignant tumor of the meibomian glands located in the eyelids. No information exists on the cytogenetic and genetic aspects of MCC. There is no report on the gene expression profile of MCC. Thus there is a need, for both scientific and clinical reasons, to identify genes and pathways that are involved in the development and progression of MCC. We analyzed the gene expression profile of MCC by the microarray technique. Forty-four genes were upregulated and 149 genes were downregulated in MCC. Differential expression data were confirmed for 5 genes by semiquantitative RT-PCR in MCC tumors: GTF2H4, RBM12, UBE2D3, DDX17, and LZTS1. We found dysregulation of two major pathways in MCC: MAPK and JAK/STAT. Clusters of genes on chromosomes 1, 12, and 19 were dysregulated in MCC. The data presented here will facilitate the identification of specific markers and therapeutic targets for the treatment of MCC patients.
AB - Meibomian cell carcinoma (MCC) is a malignant tumor of the meibomian glands located in the eyelids. No information exists on the cytogenetic and genetic aspects of MCC. There is no report on the gene expression profile of MCC. Thus there is a need, for both scientific and clinical reasons, to identify genes and pathways that are involved in the development and progression of MCC. We analyzed the gene expression profile of MCC by the microarray technique. Forty-four genes were upregulated and 149 genes were downregulated in MCC. Differential expression data were confirmed for 5 genes by semiquantitative RT-PCR in MCC tumors: GTF2H4, RBM12, UBE2D3, DDX17, and LZTS1. We found dysregulation of two major pathways in MCC: MAPK and JAK/STAT. Clusters of genes on chromosomes 1, 12, and 19 were dysregulated in MCC. The data presented here will facilitate the identification of specific markers and therapeutic targets for the treatment of MCC patients.
KW - Expression profile
KW - JAK/STAT pathway
KW - MAPK signaling pathway
KW - Meibomian cell carcinoma
KW - Microarrays
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U2 - 10.1016/j.ygeno.2007.07.008
DO - 10.1016/j.ygeno.2007.07.008
M3 - Article
C2 - 17889501
AN - SCOPUS:35549007242
SN - 0888-7543
VL - 90
SP - 559
EP - 566
JO - Genomics
JF - Genomics
IS - 5
ER -