Identification of differentially expressed serum proteins in gastric adenocarcinoma

Yashwanth Subbannayya, Sartaj Ahmad Mir, Santosh Renuse, Srikanth S. Manda, Sneha M. Pinto, Vinuth N. Puttamallesh, Hitendra Singh Solanki, H. C. Manju, Nazia Syed, Rakesh Sharma, Rita Christopher, M. Vijayakumar, K. V. Veerendra Kumar, T. S. Keshava Prasad, Girija Ramaswamy, Rekha V. Kumar, Aditi Chatterjee, Akhilesh Pandey, Harsha Gowda

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Gastric adenocarcinoma is an aggressive cancer with poor prognosis. Blood based biomarkers of gastric cancer have the potential to improve diagnosis and monitoring of these tumors. Proteins that show altered levels in the circulation of gastric cancer patients could prove useful as putative biomarkers. We used an iTRAQ-based quantitative proteomic approach to identify proteins that show altered levels in the sera of patients with gastric cancer. Our study resulted in identification of 643 proteins, of which 48 proteins showed increased levels and 11 proteins showed decreased levels in serum from gastric cancer patients compared to age and sex matched healthy controls. Proteins that showed increased expression in gastric cancer included inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), Mannose-binding protein C (MBL2), sex hormone-binding globulin (SHBG), insulin-like growth factor-binding protein 2 (IGFBP2), serum amyloid A protein (SAA1), Orosomucoid 1 (ORM1) and extracellular superoxide dismutase [Cu-Zn] (SOD3). We used multiple reaction monitoring assays and validated elevated levels of ITIH4 and SAA1 proteins in serum from gastric cancer patients. Biological significance: Gastric cancer is a highly aggressive cancer associated with high mortality. Serum-based biomarkers are of considerable interest in diagnosis and monitoring of various diseases including cancers. Gastric cancer is often diagnosed at advanced stages resulting in poor prognosis and high mortality. Pathological diagnosis using biopsy specimens remains the gold standard for diagnosis of gastric cancer. Serum-based biomarkers are of considerable importance as they are minimally invasive. In this study, we carried out quantitative proteomic profiling of serum from gastric cancer patients to identify proteins that show altered levels in gastric cancer patients. We identified more than 50 proteins that showed altered levels in gastric cancer patient sera. Validation in a large cohort of well classified patient samples would prove useful in identifying novel blood based biomarkers for gastric cancers. This article is part of a Special Issue entitled: Proteomics in India.

Original languageEnglish (US)
Pages (from-to)80-88
Number of pages9
JournalJournal of Proteomics
Volume127
DOIs
StatePublished - Sep 8 2015
Externally publishedYes

Keywords

  • Body fluid
  • In vitro labeling
  • LC-MS/MS analysis
  • Mass spectrometry
  • Serum proteome

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Fingerprint Dive into the research topics of 'Identification of differentially expressed serum proteins in gastric adenocarcinoma'. Together they form a unique fingerprint.

  • Cite this

    Subbannayya, Y., Mir, S. A., Renuse, S., Manda, S. S., Pinto, S. M., Puttamallesh, V. N., Solanki, H. S., Manju, H. C., Syed, N., Sharma, R., Christopher, R., Vijayakumar, M., Veerendra Kumar, K. V., Keshava Prasad, T. S., Ramaswamy, G., Kumar, R. V., Chatterjee, A., Pandey, A., & Gowda, H. (2015). Identification of differentially expressed serum proteins in gastric adenocarcinoma. Journal of Proteomics, 127, 80-88. https://doi.org/10.1016/j.jprot.2015.04.021