Identification of depot-specific human fat cell progenitors through distinct expression profiles and developmental gene patterns

Tamara Tchkonia, Marc Lenburg, Thomas Thomou, Nino Giorgadze, Garrett Frampton, Tamar Pirtskhalava, Andrew Cartwright, Mark Cartwright, John Flanagan, Iordanes Karagiannides, Norman Gerry, R. Armour Forse, Yourka Tchoukalova, Michael D. Jensen, Charalabos Pothoulakis, James L. Kirkland

Research output: Contribution to journalArticle

248 Scopus citations

Abstract

Anatomically separate fat depots differ in size, function, and contribution to pathological states, such as the metabolic syndrome. We isolated preadipocytes from different human fat depots to determine whether the basis for this variation is partly attributable to differences in inherent properties of fat cell progenitors. We found that genome-wide expression profiles of primary preadipocytes cultured in parallel from abdominal subcutaneous, mesenteric, and omental fat depots were distinct. Interestingly, visceral fat was not homogeneous. Preadipocytes from one of the two main visceral depots, mesenteric fat, had an expression profile closer to that of subcutaneous than omental preadipocytes, the other main visceral depot. Expression of genes that regulate early development, including homeotic genes, differed extensively among undifferentiated preadipocytes isolated from different fat depots. These profiles were confirmed by real-time PCR analysis of preadipocytes from additional lean and obese male and female subjects. We made preadipocyte strains from single abdominal subcutaneous and omental preadipocytes by expressing telomerase. Depot-specific developmental gene expression profiles persisted for 40 population doublings in these strains. Thus, human fat cell progenitors from different regions are effectively distinct, consistent with different fat depots being separate mini-organs.

Original languageEnglish (US)
Pages (from-to)E298-E307
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume292
Issue number1
DOIs
StatePublished - Jan 1 2007

Keywords

  • Homeotic genes
  • Metabolic syndrome
  • Telomerase
  • Visceral fat

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

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    Tchkonia, T., Lenburg, M., Thomou, T., Giorgadze, N., Frampton, G., Pirtskhalava, T., Cartwright, A., Cartwright, M., Flanagan, J., Karagiannides, I., Gerry, N., Forse, R. A., Tchoukalova, Y., Jensen, M. D., Pothoulakis, C., & Kirkland, J. L. (2007). Identification of depot-specific human fat cell progenitors through distinct expression profiles and developmental gene patterns. American Journal of Physiology - Endocrinology and Metabolism, 292(1), E298-E307. https://doi.org/10.1152/ajpendo.00202.2006