Identification of Class II HLA-DRB1*03-bound measles virus peptides by 2D-liquid chromatography tandem mass spectrometry

Kenneth L. Johnson; Inna G. Ovsyannikova; Gregory A. Poland; David C. Muddiman

doi:10.1021/pr0501416

Identification of Class II HLA-DRB1*03-bound measles virus peptides by 2D-liquid chromatography tandem mass spectrometry

Kenneth L. Johnson, Inna G. Ovsyannikova, Gregory A. Poland, David C. Muddiman

General Internal Medicine

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Two-dimensional liquid chromatography (2D-LC), combined with gas phase fractionation tandem mass spectrometry, was used to identify 13 naturally processed peptides originating from measles virus that were presented by HLA-DRB1*03 class II molecules. The peptides are from three of the six measles structural proteins: phosphoprotein, nucleocapsid, and hemagglutinin. These peptides provide an important first step toward understanding the mechanism of immune response to measles virus and development of a new generation of peptide-based vaccines.

Original language	English (US)
Pages (from-to)	2243-2249
Number of pages	7
Journal	Journal of Proteome Research
Volume	4
Issue number	6
DOIs	https://doi.org/10.1021/pr0501416
State	Published - Nov 2005

Keywords

2D-LC
HLA class II peptides
MS/MS
Measles
Tandem mass spectrometry

ASJC Scopus subject areas

General Chemistry
Biochemistry

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10.1021/pr0501416

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title = "Identification of Class II HLA-DRB1*03-bound measles virus peptides by 2D-liquid chromatography tandem mass spectrometry",

abstract = "Two-dimensional liquid chromatography (2D-LC), combined with gas phase fractionation tandem mass spectrometry, was used to identify 13 naturally processed peptides originating from measles virus that were presented by HLA-DRB1*03 class II molecules. The peptides are from three of the six measles structural proteins: phosphoprotein, nucleocapsid, and hemagglutinin. These peptides provide an important first step toward understanding the mechanism of immune response to measles virus and development of a new generation of peptide-based vaccines.",

keywords = "2D-LC, HLA class II peptides, MS/MS, Measles, Tandem mass spectrometry",

author = "Johnson, {Kenneth L.} and Ovsyannikova, {Inna G.} and Poland, {Gregory A.} and Muddiman, {David C.}",

year = "2005",

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AU - Johnson, Kenneth L.

AU - Ovsyannikova, Inna G.

AU - Poland, Gregory A.

AU - Muddiman, David C.

PY - 2005/11

Y1 - 2005/11

N2 - Two-dimensional liquid chromatography (2D-LC), combined with gas phase fractionation tandem mass spectrometry, was used to identify 13 naturally processed peptides originating from measles virus that were presented by HLA-DRB1*03 class II molecules. The peptides are from three of the six measles structural proteins: phosphoprotein, nucleocapsid, and hemagglutinin. These peptides provide an important first step toward understanding the mechanism of immune response to measles virus and development of a new generation of peptide-based vaccines.

AB - Two-dimensional liquid chromatography (2D-LC), combined with gas phase fractionation tandem mass spectrometry, was used to identify 13 naturally processed peptides originating from measles virus that were presented by HLA-DRB1*03 class II molecules. The peptides are from three of the six measles structural proteins: phosphoprotein, nucleocapsid, and hemagglutinin. These peptides provide an important first step toward understanding the mechanism of immune response to measles virus and development of a new generation of peptide-based vaccines.

KW - 2D-LC

KW - HLA class II peptides

KW - MS/MS

KW - Measles

KW - Tandem mass spectrometry

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Identification of Class II HLA-DRB1*03-bound measles virus peptides by 2D-liquid chromatography tandem mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

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