Identification of Class II HLA-DRB1*03-bound measles virus peptides by 2D-liquid chromatography tandem mass spectrometry

Kenneth L. Johnson, Inna G. Ovsyannikova, Gregory A. Poland, David C. Muddiman

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Two-dimensional liquid chromatography (2D-LC), combined with gas phase fractionation tandem mass spectrometry, was used to identify 13 naturally processed peptides originating from measles virus that were presented by HLA-DRB1*03 class II molecules. The peptides are from three of the six measles structural proteins: phosphoprotein, nucleocapsid, and hemagglutinin. These peptides provide an important first step toward understanding the mechanism of immune response to measles virus and development of a new generation of peptide-based vaccines.

Original languageEnglish (US)
Pages (from-to)2243-2249
Number of pages7
JournalJournal of Proteome Research
Volume4
Issue number6
DOIs
StatePublished - Nov 2005

Keywords

  • 2D-LC
  • HLA class II peptides
  • MS/MS
  • Measles
  • Tandem mass spectrometry

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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