Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entry

Masayuki Shimojima, Ute Ströher, Hideki Ebihara, Heinz Feldmann, Yoshihiro Kawaoka

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Although O-mannosylated dystroglycan is a receptor for Lassa virus, a causative agent of Lassa fever, recent findings suggest the existence of an alternative receptor(s). Here we identified four molecules as receptors for Lassa virus: Axl and Tyro3, from the TAM family, and dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) and liver and lymph node sinusoidal endothelial calcium-dependent lectin (LSECtin), from the C-type lectin family. These molecules enhanced the binding of Lassa virus to cells and mediated infection independently of dystroglycan. Axl- or Tyro3-mediated infection required intracellular signaling via the tyrosine kinase activity of Axl or Tyro3, whereas DC-SIGN- or LSECtin-mediated infection and binding were dependent on a specific carbohydrate and on ions. The identification of these four molecules as Lassa virus receptors advances our understanding of Lassa virus cell entry.

Original languageEnglish (US)
Pages (from-to)2067-2078
Number of pages12
JournalJournal of Virology
Volume86
Issue number4
DOIs
StatePublished - Feb 1 2012
Externally publishedYes

Fingerprint

Lassa virus
Dystroglycans
Virus Internalization
Cell Adhesion Molecules
dendritic cells
Dendritic Cells
receptors
lymph nodes
cells
adhesion
Lassa Fever
Infection
Lymph Nodes
infection
Calcium
Virus Receptors
calcium
Lectins
lectins
Protein-Tyrosine Kinases

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entry. / Shimojima, Masayuki; Ströher, Ute; Ebihara, Hideki; Feldmann, Heinz; Kawaoka, Yoshihiro.

In: Journal of Virology, Vol. 86, No. 4, 01.02.2012, p. 2067-2078.

Research output: Contribution to journalArticle

Shimojima, Masayuki ; Ströher, Ute ; Ebihara, Hideki ; Feldmann, Heinz ; Kawaoka, Yoshihiro. / Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entry. In: Journal of Virology. 2012 ; Vol. 86, No. 4. pp. 2067-2078.
@article{692c3bfa0f784772a2bc1df62cb30a21,
title = "Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entry",
abstract = "Although O-mannosylated dystroglycan is a receptor for Lassa virus, a causative agent of Lassa fever, recent findings suggest the existence of an alternative receptor(s). Here we identified four molecules as receptors for Lassa virus: Axl and Tyro3, from the TAM family, and dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) and liver and lymph node sinusoidal endothelial calcium-dependent lectin (LSECtin), from the C-type lectin family. These molecules enhanced the binding of Lassa virus to cells and mediated infection independently of dystroglycan. Axl- or Tyro3-mediated infection required intracellular signaling via the tyrosine kinase activity of Axl or Tyro3, whereas DC-SIGN- or LSECtin-mediated infection and binding were dependent on a specific carbohydrate and on ions. The identification of these four molecules as Lassa virus receptors advances our understanding of Lassa virus cell entry.",
author = "Masayuki Shimojima and Ute Str{\"o}her and Hideki Ebihara and Heinz Feldmann and Yoshihiro Kawaoka",
year = "2012",
month = "2",
day = "1",
doi = "10.1128/JVI.06451-11",
language = "English (US)",
volume = "86",
pages = "2067--2078",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "4",

}

TY - JOUR

T1 - Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entry

AU - Shimojima, Masayuki

AU - Ströher, Ute

AU - Ebihara, Hideki

AU - Feldmann, Heinz

AU - Kawaoka, Yoshihiro

PY - 2012/2/1

Y1 - 2012/2/1

N2 - Although O-mannosylated dystroglycan is a receptor for Lassa virus, a causative agent of Lassa fever, recent findings suggest the existence of an alternative receptor(s). Here we identified four molecules as receptors for Lassa virus: Axl and Tyro3, from the TAM family, and dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) and liver and lymph node sinusoidal endothelial calcium-dependent lectin (LSECtin), from the C-type lectin family. These molecules enhanced the binding of Lassa virus to cells and mediated infection independently of dystroglycan. Axl- or Tyro3-mediated infection required intracellular signaling via the tyrosine kinase activity of Axl or Tyro3, whereas DC-SIGN- or LSECtin-mediated infection and binding were dependent on a specific carbohydrate and on ions. The identification of these four molecules as Lassa virus receptors advances our understanding of Lassa virus cell entry.

AB - Although O-mannosylated dystroglycan is a receptor for Lassa virus, a causative agent of Lassa fever, recent findings suggest the existence of an alternative receptor(s). Here we identified four molecules as receptors for Lassa virus: Axl and Tyro3, from the TAM family, and dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) and liver and lymph node sinusoidal endothelial calcium-dependent lectin (LSECtin), from the C-type lectin family. These molecules enhanced the binding of Lassa virus to cells and mediated infection independently of dystroglycan. Axl- or Tyro3-mediated infection required intracellular signaling via the tyrosine kinase activity of Axl or Tyro3, whereas DC-SIGN- or LSECtin-mediated infection and binding were dependent on a specific carbohydrate and on ions. The identification of these four molecules as Lassa virus receptors advances our understanding of Lassa virus cell entry.

UR - http://www.scopus.com/inward/record.url?scp=84857081598&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84857081598&partnerID=8YFLogxK

U2 - 10.1128/JVI.06451-11

DO - 10.1128/JVI.06451-11

M3 - Article

VL - 86

SP - 2067

EP - 2078

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 4

ER -