Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entry

Masayuki Shimojima, Ute Ströher, Hideki Ebihara, Heinz Feldmann, Yoshihiro Kawaoka

Research output: Contribution to journalArticle

83 Scopus citations

Abstract

Although O-mannosylated dystroglycan is a receptor for Lassa virus, a causative agent of Lassa fever, recent findings suggest the existence of an alternative receptor(s). Here we identified four molecules as receptors for Lassa virus: Axl and Tyro3, from the TAM family, and dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) and liver and lymph node sinusoidal endothelial calcium-dependent lectin (LSECtin), from the C-type lectin family. These molecules enhanced the binding of Lassa virus to cells and mediated infection independently of dystroglycan. Axl- or Tyro3-mediated infection required intracellular signaling via the tyrosine kinase activity of Axl or Tyro3, whereas DC-SIGN- or LSECtin-mediated infection and binding were dependent on a specific carbohydrate and on ions. The identification of these four molecules as Lassa virus receptors advances our understanding of Lassa virus cell entry.

Original languageEnglish (US)
Pages (from-to)2067-2078
Number of pages12
JournalJournal of virology
Volume86
Issue number4
DOIs
StatePublished - Feb 2012

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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