Identification of bile acid coenzyme A synthetase in rat kidney

J. B. Kwakye, S. Barnes, Robert B Diasio

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Bile acid CoA synthetase has been discovered in rat kidney. Incubation of kidney microsomes with [14C]chenodeoxycholic acid and CoA produced a single peak with the high performance liquid chromatography (HPLC) retention time of CDC-CoA. This peak, when incubated with purified bile acid CoA:amino acid N- acyltransferase (BAT) from human liver and either taurine or glycine, led to the formation of CDC-taurine or CDC-glycine, respectively. Kinetic analysis revealed apparent K(m)s for CDC and CoA of 2.5 μM and 2.6 μM, respectively. This activity appeared specific for bile acids as it was not inhibited by benzoic acid or salicylic acid, known substrates for other rat kidney CoA synthetases. This demonstrates that the kidney has the potential for bile acid metabolism and may have a role in bile acid physiology.

Original languageEnglish (US)
Pages (from-to)95-99
Number of pages5
JournalJournal of Lipid Research
Volume34
Issue number1
StatePublished - 1993
Externally publishedYes

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Coenzyme A Ligases
Coenzyme A
Centers for Disease Control and Prevention (U.S.)
Bile Acids and Salts
Rats
Kidney
Taurine
Glycine
Chenodeoxycholic Acid
Benzoic Acid
Salicylic Acid
Physiology
High performance liquid chromatography
Ligases
Microsomes
Metabolism
Liver
High Pressure Liquid Chromatography
Kinetics
Substrates

Keywords

  • chenodeoxycholyl- CoA
  • chenodeoxycholylglycine
  • chenodeoxycholyltaurine
  • microsomes

ASJC Scopus subject areas

  • Endocrinology

Cite this

Identification of bile acid coenzyme A synthetase in rat kidney. / Kwakye, J. B.; Barnes, S.; Diasio, Robert B.

In: Journal of Lipid Research, Vol. 34, No. 1, 1993, p. 95-99.

Research output: Contribution to journalArticle

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AU - Diasio, Robert B

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