Identification of bile acid-CoA: amino acid N-acyltransferase in rat kidney

J. B. Kwakye, M. R. Johnson, S. Barnes, W. E. Grizzle, Robert B Diasio

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A novel location of the bile-acid-conjugating enzyme bile acid-CoA:amino acid N-acyltransferase (BAT) has been discovered in the cytosolic fraction of rat kidney. Both taurine and glycine were utilized as substrates. Formation of bile acid N-acyl amidates was verified by h.p.l.c. by comparison with authentic standards and by specific hydrolysis using cholylglycine hydrolase. Immunoblot analysis using a human liver anti-BAT polyclonal antibody indicated that rat kidney BAT has the same molecular mass as rat liver BAT. These findings suggest that the kidney has a role in bile acid metabolism and physiology.

Original languageEnglish (US)
Pages (from-to)821-824
Number of pages4
JournalBiochemical Journal
Volume280
Issue number3
StatePublished - 1991
Externally publishedYes

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Bile Acids and Salts
Rats
choloylglycine hydrolase
Kidney
Liver
Taurine
Physiology
Molecular mass
Metabolism
Glycine
Hydrolysis
Antibodies
Substrates
Enzymes
bile acid-CoA amino acid N-acyltransferase

ASJC Scopus subject areas

  • Biochemistry

Cite this

Kwakye, J. B., Johnson, M. R., Barnes, S., Grizzle, W. E., & Diasio, R. B. (1991). Identification of bile acid-CoA: amino acid N-acyltransferase in rat kidney. Biochemical Journal, 280(3), 821-824.

Identification of bile acid-CoA : amino acid N-acyltransferase in rat kidney. / Kwakye, J. B.; Johnson, M. R.; Barnes, S.; Grizzle, W. E.; Diasio, Robert B.

In: Biochemical Journal, Vol. 280, No. 3, 1991, p. 821-824.

Research output: Contribution to journalArticle

Kwakye, JB, Johnson, MR, Barnes, S, Grizzle, WE & Diasio, RB 1991, 'Identification of bile acid-CoA: amino acid N-acyltransferase in rat kidney', Biochemical Journal, vol. 280, no. 3, pp. 821-824.
Kwakye, J. B. ; Johnson, M. R. ; Barnes, S. ; Grizzle, W. E. ; Diasio, Robert B. / Identification of bile acid-CoA : amino acid N-acyltransferase in rat kidney. In: Biochemical Journal. 1991 ; Vol. 280, No. 3. pp. 821-824.
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