Identification of androgen receptors in normal human osteoblast-like cells

D. S. Colvard, E. F. Eriksen, P. E. Keeting, E. M. Wilson, D. B. Lubahn, F. S. French, B. L. Riggs, T. C. Spelsberg

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Abstract

The sex steroids, androgens and estrogens, are major regulators of bone metabolism. However, whether these hormones act on bone cells through direct or indirect mechanisms has remained unclear. A nuclear binding assay recently used to demonstrate estrogen receptors in bone [Eriksen, E.F., Colvard, D.S., Berg, N.J., Graham, M.L., Mann, K.G., Spelsberg, T.C. & Riggs, B.L. (1988) Science 241, 84-86] was used to identify specific nuclear binding of a tritiated synthetic androgen, [3H]R1881 (methyltrienolone), in 21 of 25 (84%) human osteoblast-like cell strains and a concentration of bound steroid receptors of 821 ± 140 (mean ± SEM) molecules per cell nucleus. Binding was saturable and steroid-specific. Androgen receptor gene expression in osteoblasts was confirmed by RNA blot analysis. Relative concentrations of androgen and estrogen receptors were compared by measuring specific nuclear estrogen binding. Nuclear binding of [3H]estradiol was observed in 27 of 30 (90%) cell strains; the concentration of bound estradiol receptor was 1537 ± 221 molecules per cell nucleus. The concentrations of nuclear binding sites were similar in males and females for both [3H]R1881 and [3H]estradiol. We conclude that both androgens and estrogens act directly on human bone cells through their respective receptor-mediated mechanisms.

Original languageEnglish (US)
Pages (from-to)854-857
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number3
DOIs
StatePublished - 1989

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    Colvard, D. S., Eriksen, E. F., Keeting, P. E., Wilson, E. M., Lubahn, D. B., French, F. S., Riggs, B. L., & Spelsberg, T. C. (1989). Identification of androgen receptors in normal human osteoblast-like cells. Proceedings of the National Academy of Sciences of the United States of America, 86(3), 854-857. https://doi.org/10.1073/pnas.86.3.854