Identification of acidic dileucine signals in LRP9 that interact with both GGAs and AP-1/AP-2

Balraj Doray, Jane M. Knisely, Lukas Wartman, Guojun Bu, Stuart Kornfeld

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

The Golgi-localized, gamma-ear-containing, ADP ribosylation factor-binding family of monomeric clathrin adaptors (GGAs) is known to bind cargo molecules through short C-terminal peptide motifs conforming to the sequence DXXLL (X=any amino acid), while the heterotetrameric adaptors AP-1 and AP-2 utilize a similar but discrete sorting motif of the sequence [D,E]XXXL[L,I]. While it has been established that a single cargo molecule may contain either or both types of these acidic cluster-dileucine (AC-LL) sorting signals, there are no examples of cargo with overlapping GGA and AP-1/AP-2-binding motifs. In this study, we report that the cytosolic tail of low-density lipoprotein receptor-related protein (LRP)9 contains a bifunctional GGA and AP-1/ AP-2-binding motif at its carboxy-terminus (EDEPLL). We further demonstrate that the internal EDEVLL sequence of LRP9 also binds to GGAs in addition to AP-2. Either AC-LL motif of LRP9 is functional in endocytosis. These findings represent the first study characterizing the trafficking of LRP9 and also have implications for the identification of additional GGA cargo molecules.

Original languageEnglish (US)
Pages (from-to)1551-1562
Number of pages12
JournalTraffic
Volume9
Issue number9
DOIs
StatePublished - 2008

Keywords

  • AP-1
  • AP-2
  • Dileucine motif
  • Endocytosis
  • GGAs
  • LRP9

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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