Identification of a polymorphism in the human neurotensin receptor gene

M. Watson, P. J. Isackson, M. Makker, M. S. Yamada, M. Yamada, B. Cusack, E. Richelson

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

A complementary DNA (cDNA) clone encoding the neurotensin receptor was isolated from a human substantia nigra cDNA library. The deduced amino acid sequence of this clone was almost identical to that of a cDNA for this receptor cloned from a previously described HT29 human colonic adenocarcinoma cell line. We found three base changes between the previously reported HT29 cDNA clone and the current cDNA clone. We investigated these changes by using polymerase chain reactions to amplify these areas from various human samples. One of the differences, which resulted in an amino acid change at AA194 (a leucine in the HT29 sequence was a phenylalanine in the current sequence), was found in some, but not in all, human samples. This finding represents genetic variability in human neurotensin receptors, the first such report for a peptide receptor. Both of these receptors, however, when expressed separately in transfected cell lines, had similar affinities for neurotensin and some related peptides.

Original languageEnglish (US)
Pages (from-to)1043-1048
Number of pages6
JournalMayo Clinic Proceedings
Volume68
Issue number11
StatePublished - 1993

Fingerprint

Neurotensin Receptors
Complementary DNA
Clone Cells
Genes
Cell Line
Neurotensin
Peptide Receptors
Substantia Nigra
Phenylalanine
Gene Library
Leucine
Amino Acid Sequence
Adenocarcinoma
Amino Acids
Polymerase Chain Reaction
Peptides

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Watson, M., Isackson, P. J., Makker, M., Yamada, M. S., Yamada, M., Cusack, B., & Richelson, E. (1993). Identification of a polymorphism in the human neurotensin receptor gene. Mayo Clinic Proceedings, 68(11), 1043-1048.

Identification of a polymorphism in the human neurotensin receptor gene. / Watson, M.; Isackson, P. J.; Makker, M.; Yamada, M. S.; Yamada, M.; Cusack, B.; Richelson, E.

In: Mayo Clinic Proceedings, Vol. 68, No. 11, 1993, p. 1043-1048.

Research output: Contribution to journalArticle

Watson, M, Isackson, PJ, Makker, M, Yamada, MS, Yamada, M, Cusack, B & Richelson, E 1993, 'Identification of a polymorphism in the human neurotensin receptor gene', Mayo Clinic Proceedings, vol. 68, no. 11, pp. 1043-1048.
Watson M, Isackson PJ, Makker M, Yamada MS, Yamada M, Cusack B et al. Identification of a polymorphism in the human neurotensin receptor gene. Mayo Clinic Proceedings. 1993;68(11):1043-1048.
Watson, M. ; Isackson, P. J. ; Makker, M. ; Yamada, M. S. ; Yamada, M. ; Cusack, B. ; Richelson, E. / Identification of a polymorphism in the human neurotensin receptor gene. In: Mayo Clinic Proceedings. 1993 ; Vol. 68, No. 11. pp. 1043-1048.
@article{d62249fe9378433e9b8561dded40eab7,
title = "Identification of a polymorphism in the human neurotensin receptor gene",
abstract = "A complementary DNA (cDNA) clone encoding the neurotensin receptor was isolated from a human substantia nigra cDNA library. The deduced amino acid sequence of this clone was almost identical to that of a cDNA for this receptor cloned from a previously described HT29 human colonic adenocarcinoma cell line. We found three base changes between the previously reported HT29 cDNA clone and the current cDNA clone. We investigated these changes by using polymerase chain reactions to amplify these areas from various human samples. One of the differences, which resulted in an amino acid change at AA194 (a leucine in the HT29 sequence was a phenylalanine in the current sequence), was found in some, but not in all, human samples. This finding represents genetic variability in human neurotensin receptors, the first such report for a peptide receptor. Both of these receptors, however, when expressed separately in transfected cell lines, had similar affinities for neurotensin and some related peptides.",
author = "M. Watson and Isackson, {P. J.} and M. Makker and Yamada, {M. S.} and M. Yamada and B. Cusack and E. Richelson",
year = "1993",
language = "English (US)",
volume = "68",
pages = "1043--1048",
journal = "Mayo Clinic Proceedings",
issn = "0025-6196",
publisher = "Elsevier Science",
number = "11",

}

TY - JOUR

T1 - Identification of a polymorphism in the human neurotensin receptor gene

AU - Watson, M.

AU - Isackson, P. J.

AU - Makker, M.

AU - Yamada, M. S.

AU - Yamada, M.

AU - Cusack, B.

AU - Richelson, E.

PY - 1993

Y1 - 1993

N2 - A complementary DNA (cDNA) clone encoding the neurotensin receptor was isolated from a human substantia nigra cDNA library. The deduced amino acid sequence of this clone was almost identical to that of a cDNA for this receptor cloned from a previously described HT29 human colonic adenocarcinoma cell line. We found three base changes between the previously reported HT29 cDNA clone and the current cDNA clone. We investigated these changes by using polymerase chain reactions to amplify these areas from various human samples. One of the differences, which resulted in an amino acid change at AA194 (a leucine in the HT29 sequence was a phenylalanine in the current sequence), was found in some, but not in all, human samples. This finding represents genetic variability in human neurotensin receptors, the first such report for a peptide receptor. Both of these receptors, however, when expressed separately in transfected cell lines, had similar affinities for neurotensin and some related peptides.

AB - A complementary DNA (cDNA) clone encoding the neurotensin receptor was isolated from a human substantia nigra cDNA library. The deduced amino acid sequence of this clone was almost identical to that of a cDNA for this receptor cloned from a previously described HT29 human colonic adenocarcinoma cell line. We found three base changes between the previously reported HT29 cDNA clone and the current cDNA clone. We investigated these changes by using polymerase chain reactions to amplify these areas from various human samples. One of the differences, which resulted in an amino acid change at AA194 (a leucine in the HT29 sequence was a phenylalanine in the current sequence), was found in some, but not in all, human samples. This finding represents genetic variability in human neurotensin receptors, the first such report for a peptide receptor. Both of these receptors, however, when expressed separately in transfected cell lines, had similar affinities for neurotensin and some related peptides.

UR - http://www.scopus.com/inward/record.url?scp=0027144468&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027144468&partnerID=8YFLogxK

M3 - Article

VL - 68

SP - 1043

EP - 1048

JO - Mayo Clinic Proceedings

JF - Mayo Clinic Proceedings

SN - 0025-6196

IS - 11

ER -