Identification of a Polymorphism in the Human Neurotensin Receptor Gene

MICHAEL WATSON, PAUL J. ISACKSON, MANISHA MAKKER, MITSUHIKO S. YAMADA, MISA YAMADA, BERNADETTE CUSACK, ELLIOTT RICHELSON

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

A complementary DNA (cDNA) clone encoding the neurotensin receptor was isolated from a human substantia nigra cDNA library. The deduced amino acid sequence of this clone was almost identical to that of a cDNA for this receptor cloned from a previously described HT29 human colonic adenocarcinoma cell line. We found three base changes between the previously reported HT29 cDNA clone and the current cDNA clone. We investigated these changes by using polymerase chain reactions to amplify these areas from various human samples. One of the differences, which resulted in an amino acid change at AA194 (a leucine in the HT29 sequence was a phenylalanine in the current sequence), was found in some, but not in all, human samples. This finding represents genetic variability in human neurotensin receptors, the first such report for a peptide receptor. Both of these receptors, however, when expressed separately in transfected cell lines, had similar affinities for neurotensin and some related peptides.

Original languageEnglish (US)
Pages (from-to)1043-1048
Number of pages6
JournalMayo Clinic proceedings
Volume68
Issue number11
DOIs
StatePublished - 1993

ASJC Scopus subject areas

  • Medicine(all)

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