TY - JOUR
T1 - Identification of a Polymorphism in the Human Neurotensin Receptor Gene
AU - WATSON, MICHAEL
AU - ISACKSON, PAUL J.
AU - MAKKER, MANISHA
AU - YAMADA, MITSUHIKO S.
AU - YAMADA, MISA
AU - CUSACK, BERNADETTE
AU - RICHELSON, ELLIOTT
N1 - Funding Information:
This study was supported in part by Grant MH 27692 from the National Institutes of Health, Public Health Service, and by the Mayo Foundation.
PY - 1993
Y1 - 1993
N2 - A complementary DNA (cDNA) clone encoding the neurotensin receptor was isolated from a human substantia nigra cDNA library. The deduced amino acid sequence of this clone was almost identical to that of a cDNA for this receptor cloned from a previously described HT29 human colonic adenocarcinoma cell line. We found three base changes between the previously reported HT29 cDNA clone and the current cDNA clone. We investigated these changes by using polymerase chain reactions to amplify these areas from various human samples. One of the differences, which resulted in an amino acid change at AA194 (a leucine in the HT29 sequence was a phenylalanine in the current sequence), was found in some, but not in all, human samples. This finding represents genetic variability in human neurotensin receptors, the first such report for a peptide receptor. Both of these receptors, however, when expressed separately in transfected cell lines, had similar affinities for neurotensin and some related peptides.
AB - A complementary DNA (cDNA) clone encoding the neurotensin receptor was isolated from a human substantia nigra cDNA library. The deduced amino acid sequence of this clone was almost identical to that of a cDNA for this receptor cloned from a previously described HT29 human colonic adenocarcinoma cell line. We found three base changes between the previously reported HT29 cDNA clone and the current cDNA clone. We investigated these changes by using polymerase chain reactions to amplify these areas from various human samples. One of the differences, which resulted in an amino acid change at AA194 (a leucine in the HT29 sequence was a phenylalanine in the current sequence), was found in some, but not in all, human samples. This finding represents genetic variability in human neurotensin receptors, the first such report for a peptide receptor. Both of these receptors, however, when expressed separately in transfected cell lines, had similar affinities for neurotensin and some related peptides.
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U2 - 10.1016/S0025-6196(12)60896-9
DO - 10.1016/S0025-6196(12)60896-9
M3 - Article
C2 - 8231267
AN - SCOPUS:0027144468
SN - 0025-6196
VL - 68
SP - 1043
EP - 1048
JO - Mayo Clinic proceedings
JF - Mayo Clinic proceedings
IS - 11
ER -