Identification of a polymorphic nucleotide in oxyR specific for Mycobacterium bovis

Srinand Sreevatsan, Patricio Escalante, X. Pan, Duncan A. Gillies, Subeeh Siddiqui, Christina N. Khalaf, Barry N. Kreiswirth, Pablo Bifani, L. Garry Adams, Thomas Ficht, Veera S. Perumaalla, M. Donald Cave, Jan D A Van Embden, James M. Musser

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Automated sequence analysis of a 410-bp region of the oxyR gene in 105 Mycobacterium tuberculosis complex isolates identified a polymorphic nucleotide that differentiated Mycobacterium bovis isolates from other complex members. All 29 M. bovis isolates sequenced had an adenine residue at nucleotide 285, whereas all 76 other complex isolates had a guanine residue. PCR-restriction fragment length polymorphism analysis of oxyR with restriction endonuclease AluI in an additional 255 complex isolates from widespread intercontinental sources confirmed and extended the unique association of adenine at position 285 with M. bovis isolates.

Original languageEnglish (US)
Pages (from-to)2007-2010
Number of pages4
JournalJournal of Clinical Microbiology
Volume34
Issue number8
StatePublished - Aug 1996
Externally publishedYes

Fingerprint

Mycobacterium bovis
Nucleotides
Adenine
DNA Restriction Enzymes
Guanine
Mycobacterium tuberculosis
Restriction Fragment Length Polymorphisms
Sequence Analysis
Polymerase Chain Reaction
Genes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Sreevatsan, S., Escalante, P., Pan, X., Gillies, D. A., Siddiqui, S., Khalaf, C. N., ... Musser, J. M. (1996). Identification of a polymorphic nucleotide in oxyR specific for Mycobacterium bovis. Journal of Clinical Microbiology, 34(8), 2007-2010.

Identification of a polymorphic nucleotide in oxyR specific for Mycobacterium bovis. / Sreevatsan, Srinand; Escalante, Patricio; Pan, X.; Gillies, Duncan A.; Siddiqui, Subeeh; Khalaf, Christina N.; Kreiswirth, Barry N.; Bifani, Pablo; Adams, L. Garry; Ficht, Thomas; Perumaalla, Veera S.; Cave, M. Donald; Van Embden, Jan D A; Musser, James M.

In: Journal of Clinical Microbiology, Vol. 34, No. 8, 08.1996, p. 2007-2010.

Research output: Contribution to journalArticle

Sreevatsan, S, Escalante, P, Pan, X, Gillies, DA, Siddiqui, S, Khalaf, CN, Kreiswirth, BN, Bifani, P, Adams, LG, Ficht, T, Perumaalla, VS, Cave, MD, Van Embden, JDA & Musser, JM 1996, 'Identification of a polymorphic nucleotide in oxyR specific for Mycobacterium bovis', Journal of Clinical Microbiology, vol. 34, no. 8, pp. 2007-2010.
Sreevatsan S, Escalante P, Pan X, Gillies DA, Siddiqui S, Khalaf CN et al. Identification of a polymorphic nucleotide in oxyR specific for Mycobacterium bovis. Journal of Clinical Microbiology. 1996 Aug;34(8):2007-2010.
Sreevatsan, Srinand ; Escalante, Patricio ; Pan, X. ; Gillies, Duncan A. ; Siddiqui, Subeeh ; Khalaf, Christina N. ; Kreiswirth, Barry N. ; Bifani, Pablo ; Adams, L. Garry ; Ficht, Thomas ; Perumaalla, Veera S. ; Cave, M. Donald ; Van Embden, Jan D A ; Musser, James M. / Identification of a polymorphic nucleotide in oxyR specific for Mycobacterium bovis. In: Journal of Clinical Microbiology. 1996 ; Vol. 34, No. 8. pp. 2007-2010.
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