Identification of a novel splicing isoform of murine CGI-58

Xingyuan Yang, Xin Lu, Jun Liu

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The comparative gene identification-58 (CGI-58) gene, mutations of which are linked to Chanarin-Dorfman syndrome, encodes a protein of the α/β hydrolase domain subfamily. We report here a new alternative splicing isoform of the murine CGI-58 gene, termed mCGI-58S. Sequence comparison indicates the lack of second and third exons in this cDNA variant. While the full-length protein displayed perilipin-dependent localization to lipid droplets, mCGI-58S showed a predominant cytoplasmic staining when expressed in cells. mCGI-58S was incapable of activating adipose triglyceride lipase but retained the capacity to acylate lysophosphatidic acid. Overexpression of mCGI-58S failed to promote lipid droplet turnover and loss of intracellular triacylglycerols. These results suggest that this splicing event may be involved in the regulation of lipid homeostasis.

Original languageEnglish (US)
Pages (from-to)903-910
Number of pages8
JournalFEBS Letters
Volume584
Issue number5
DOIs
StatePublished - Mar 5 2010

Keywords

  • Lipid droplet
  • Lipolysis
  • Lysophosphatidic acid
  • Neutral lipid storage disease
  • Triacylglycerol

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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