Abstract
Previous studies directed towards identifying epitopes on type II collagen (CII) important in collagen induced arthritis (CIA) in mice have focused primarily on responses mounted in susceptible H-2q strains. However, the nature of T and B cell responses against CII in susceptible H-2r strains remains ill-defined. In an effort to identify regions on CII important in CIA in H-2r mice, we examined the cellular and humoral response of susceptible B10.RIII (H-2r) mice against cyanogen bromide (CB)-cleaved fragments of porcine CII. Following immunization with native porcine CII, LNC from B10.RIII mice mounted proliferative responses predominantly to peptide CB10, while negligible proliferation was detected against fragment CB9,7, CB8, CB11 or CB12. In contrast, sera from arthritic B10.RIII mice displayed a heterogeneous pattern of reactivity against porcine CII, with strong antibody binding measured against the major fragments CB11, CB8 and CB10. To determine the in vivo significance of the dominant cellular response to CB10, B10.RIII mice received an i.v. injection of soluble CB10 seven days before immunization with native procine CII. Mice pretreated with CB10 were highly resistant to CIA compared to control animals. Interestingly, B10. RIII mice pretreated with fragment CB11, a region of CII implicated in H-2q-restricted CIA, remained susceptible to arthritis induction. Collectively, our findings indicate that the CB10 region of porcine C11 bears determinants which may be important in the induction and/or regulation of CIA in the H-2r haplotype.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 39-49 |
| Number of pages | 11 |
| Journal | Autoimmunity |
| Volume | 20 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1995 |
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Keywords
- Collagen Induced Arthritis
- T Cell Epitopes
- Type II Collagen
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
Identification of a cyanogen bromide fragment of porcine type II collagen capable of modulating collagen arthritis in b10.RIII (h-2r) mice. / Nabozny, Gerald H.; Griffiths, Marie M.; Harper, D. Scott; Luthra, Harvinder S.; David, Chella S.
In: Autoimmunity, Vol. 20, No. 1, 1995, p. 39-49.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of a cyanogen bromide fragment of porcine type II collagen capable of modulating collagen arthritis in b10.RIII (h-2r) mice
AU - Nabozny, Gerald H.
AU - Griffiths, Marie M.
AU - Harper, D. Scott
AU - Luthra, Harvinder S.
AU - David, Chella S.
PY - 1995
Y1 - 1995
N2 - Previous studies directed towards identifying epitopes on type II collagen (CII) important in collagen induced arthritis (CIA) in mice have focused primarily on responses mounted in susceptible H-2q strains. However, the nature of T and B cell responses against CII in susceptible H-2r strains remains ill-defined. In an effort to identify regions on CII important in CIA in H-2r mice, we examined the cellular and humoral response of susceptible B10.RIII (H-2r) mice against cyanogen bromide (CB)-cleaved fragments of porcine CII. Following immunization with native porcine CII, LNC from B10.RIII mice mounted proliferative responses predominantly to peptide CB10, while negligible proliferation was detected against fragment CB9,7, CB8, CB11 or CB12. In contrast, sera from arthritic B10.RIII mice displayed a heterogeneous pattern of reactivity against porcine CII, with strong antibody binding measured against the major fragments CB11, CB8 and CB10. To determine the in vivo significance of the dominant cellular response to CB10, B10.RIII mice received an i.v. injection of soluble CB10 seven days before immunization with native procine CII. Mice pretreated with CB10 were highly resistant to CIA compared to control animals. Interestingly, B10. RIII mice pretreated with fragment CB11, a region of CII implicated in H-2q-restricted CIA, remained susceptible to arthritis induction. Collectively, our findings indicate that the CB10 region of porcine C11 bears determinants which may be important in the induction and/or regulation of CIA in the H-2r haplotype.
AB - Previous studies directed towards identifying epitopes on type II collagen (CII) important in collagen induced arthritis (CIA) in mice have focused primarily on responses mounted in susceptible H-2q strains. However, the nature of T and B cell responses against CII in susceptible H-2r strains remains ill-defined. In an effort to identify regions on CII important in CIA in H-2r mice, we examined the cellular and humoral response of susceptible B10.RIII (H-2r) mice against cyanogen bromide (CB)-cleaved fragments of porcine CII. Following immunization with native porcine CII, LNC from B10.RIII mice mounted proliferative responses predominantly to peptide CB10, while negligible proliferation was detected against fragment CB9,7, CB8, CB11 or CB12. In contrast, sera from arthritic B10.RIII mice displayed a heterogeneous pattern of reactivity against porcine CII, with strong antibody binding measured against the major fragments CB11, CB8 and CB10. To determine the in vivo significance of the dominant cellular response to CB10, B10.RIII mice received an i.v. injection of soluble CB10 seven days before immunization with native procine CII. Mice pretreated with CB10 were highly resistant to CIA compared to control animals. Interestingly, B10. RIII mice pretreated with fragment CB11, a region of CII implicated in H-2q-restricted CIA, remained susceptible to arthritis induction. Collectively, our findings indicate that the CB10 region of porcine C11 bears determinants which may be important in the induction and/or regulation of CIA in the H-2r haplotype.
KW - Collagen Induced Arthritis
KW - T Cell Epitopes
KW - Type II Collagen
UR - http://www.scopus.com/inward/record.url?scp=0029022895&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029022895&partnerID=8YFLogxK
U2 - 10.3109/08916939508993338
DO - 10.3109/08916939508993338
M3 - Article
VL - 20
SP - 39
EP - 49
JO - Autoimmunity
JF - Autoimmunity
SN - 0891-6934
IS - 1
ER -