Identification of γ1 subunit of GABAA receptor in rat testis

Jinghua Hu, Yuan Chang Yan

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The isoform type of γ subunits of GABAA receptor is a molecular determinant of its pharmacological characteristics. At present, the existence of GABAA receptor in mammalian sperm is still a controversy. By using degenerate primers designed according to highly conserved region in all three γ (γ1, γ2 and γ3) subunits cloned in rat brain, we performed reverse transcription polymerase chain reaction (RT-PCR) to examine the expression pattern of γ subunits of GABAA receptor in rat testis. Only one 370 bp fragment was obtained from RT-PCR in rat testis and sequencing results showed that it represented γ1 subunit, but not γ2 or γ3 subunit. Using the cloned fragment as probe, a 3.8 kb transcript which in size as same as γ1 subunit in rat brain was detected in rat testis mRNA by performing Northern blot assay. Furthermore, results of in situ hybridization assay confirmed that γ1 subunit was expressed in round spermatids and spermatozoa, maybe also in secondary spermatocyte. These evidences proved that γ1 subunit of GABAA receptor is exclusively expressed in rat testis and this feature may be the structural basis of the specific function of GABAA receptors in sperm acrosome reaction.

Original languageEnglish (US)
Pages (from-to)33-37
Number of pages5
JournalCell Research
Volume12
Issue number1
StatePublished - Mar 2002
Externally publishedYes

Fingerprint

GABA-A Receptors
Testis
Spermatozoa
Reverse Transcription
Acrosome Reaction
Polymerase Chain Reaction
Spermatocytes
Spermatids
Brain
Northern Blotting
In Situ Hybridization
Protein Isoforms
Pharmacology
Messenger RNA

Keywords

  • γ subunit
  • GABA receptor
  • Rat testis

ASJC Scopus subject areas

  • Cell Biology

Cite this

Identification of γ1 subunit of GABAA receptor in rat testis. / Hu, Jinghua; Yan, Yuan Chang.

In: Cell Research, Vol. 12, No. 1, 03.2002, p. 33-37.

Research output: Contribution to journalArticle

Hu, Jinghua ; Yan, Yuan Chang. / Identification of γ1 subunit of GABAA receptor in rat testis. In: Cell Research. 2002 ; Vol. 12, No. 1. pp. 33-37.
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