Identification of γ-aminobutyric acid transporter (GAT1) on the rat sperm

Jinghua Hu, Xiao Bing He, Yuan Chang Yan

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Some recent studies indicated that GABAergic system is involved in mammalian sperm acrosome reaction (AR), but direct evidence pertaining to the expression of gat1 in mammalian sperm is not yet demonstrated. In this study, we evaluated the presence of 67kDa GAT1 protein and mRNA in rat testis by Western blotting and reverse transcription-polymerase chain reaction. Meanwhile, immunohistochemical and immunofluorescent analyses also identified GAT1 protein on the elongated spermatid and sperm. These results indicated that rat testis is a novel site of gat1 expression. Further studies should be taken to explore the role of GAT1 protein on sperm acrosome reaction.

Original languageEnglish (US)
Pages (from-to)51-58
Number of pages8
JournalCell Research
Volume10
Issue number1
StatePublished - Mar 2000
Externally publishedYes

Fingerprint

GABA Plasma Membrane Transport Proteins
Aminobutyrates
Spermatozoa
Acrosome Reaction
Testis
Proteins
Spermatids
Reverse Transcription
Western Blotting
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Expression
  • Gat1
  • Rat testis
  • Sperm

ASJC Scopus subject areas

  • Cell Biology

Cite this

Identification of γ-aminobutyric acid transporter (GAT1) on the rat sperm. / Hu, Jinghua; He, Xiao Bing; Yan, Yuan Chang.

In: Cell Research, Vol. 10, No. 1, 03.2000, p. 51-58.

Research output: Contribution to journalArticle

Hu, Jinghua ; He, Xiao Bing ; Yan, Yuan Chang. / Identification of γ-aminobutyric acid transporter (GAT1) on the rat sperm. In: Cell Research. 2000 ; Vol. 10, No. 1. pp. 51-58.
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