Identification of γ-aminobutyric acid transporter (GAT1) on the rat sperm

Jing Hua Hu; Xiao Bing He; Yuan Chang Yan

doi:10.1038/sj.cr.7290035

Identification of γ-aminobutyric acid transporter (GAT1) on the rat sperm

Jing Hua Hu, Xiao Bing He, Yuan Chang Yan

Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Some recent studies indicated that GABAergic system is involved in mammalian sperm acrosome reaction (AR), but direct evidence pertaining to the expression of gat1 in mammalian sperm is not yet demonstrated. In this study, we evaluated the presence of 67kDa GAT1 protein and mRNA in rat testis by Western blotting and reverse transcription-polymerase chain reaction. Meanwhile, immunohistochemical and immunofluorescent analyses also identified GAT1 protein on the elongated spermatid and sperm. These results indicated that rat testis is a novel site of gat1 expression. Further studies should be taken to explore the role of GAT1 protein on sperm acrosome reaction.

Original language	English (US)
Pages (from-to)	51-58
Number of pages	8
Journal	Cell Research
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/sj.cr.7290035
State	Published - Mar 2000

Keywords

Expression
Gat1
Rat testis
Sperm

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1038/sj.cr.7290035

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abstract = "Some recent studies indicated that GABAergic system is involved in mammalian sperm acrosome reaction (AR), but direct evidence pertaining to the expression of gat1 in mammalian sperm is not yet demonstrated. In this study, we evaluated the presence of 67kDa GAT1 protein and mRNA in rat testis by Western blotting and reverse transcription-polymerase chain reaction. Meanwhile, immunohistochemical and immunofluorescent analyses also identified GAT1 protein on the elongated spermatid and sperm. These results indicated that rat testis is a novel site of gat1 expression. Further studies should be taken to explore the role of GAT1 protein on sperm acrosome reaction.",

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N2 - Some recent studies indicated that GABAergic system is involved in mammalian sperm acrosome reaction (AR), but direct evidence pertaining to the expression of gat1 in mammalian sperm is not yet demonstrated. In this study, we evaluated the presence of 67kDa GAT1 protein and mRNA in rat testis by Western blotting and reverse transcription-polymerase chain reaction. Meanwhile, immunohistochemical and immunofluorescent analyses also identified GAT1 protein on the elongated spermatid and sperm. These results indicated that rat testis is a novel site of gat1 expression. Further studies should be taken to explore the role of GAT1 protein on sperm acrosome reaction.

AB - Some recent studies indicated that GABAergic system is involved in mammalian sperm acrosome reaction (AR), but direct evidence pertaining to the expression of gat1 in mammalian sperm is not yet demonstrated. In this study, we evaluated the presence of 67kDa GAT1 protein and mRNA in rat testis by Western blotting and reverse transcription-polymerase chain reaction. Meanwhile, immunohistochemical and immunofluorescent analyses also identified GAT1 protein on the elongated spermatid and sperm. These results indicated that rat testis is a novel site of gat1 expression. Further studies should be taken to explore the role of GAT1 protein on sperm acrosome reaction.

KW - Expression

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KW - Sperm

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Identification of γ-aminobutyric acid transporter (GAT1) on the rat sperm

Abstract

Keywords

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