Identification and transcriptional analysis of the homologues of the herpes simplex virus type 1 UL30 to UL40 genes in the genome of nononcogenic Marek's disease virus serotype 2

Yoshihiro Izumiya, Hyung Kwan Jang, Mie Sugawara, Yasuhiro H Ikeda, Ryuichi Miura, Yorihiro Nishimura, Kazuya Nakamura, Takayuki Miyazawa, Chieko Kai, Takeshi Mikami

Research output: Contribution to journalArticle

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Abstract

Studies on Marek's disease virus serotype 2 (MDV2) are important for understanding the natural nononcogenic phenotype of MDV. This study reports a 27,535 bp nucleotide sequence of part of the MDV2 genome located in the central unique long (U(L)) region. The analysis revealed 11 complete ORFs with high amino acid sequence identities to the products of other alphaherpesviruses. The MDV2 ORFs were arranged collinearly with the prototype sequence of herpes simplex virus type 1, ranging from the UL30 to UL40 genes. Sequences that were particularly well conserved among alphaherpesviruses were the putative functional domain of the DNA polymerase (UL30) and the ribonucleotide reductase large and small subunits (UL39 and UL40). On the other hand, in contrast to oncogenic MDV1, MDV2 did not contain the conserved proline-repeat region in the UL36 homologue. All the genes identified were confirmed to be transcribed as 3'-coterminal mRNAs and/or unique transcripts in virus-infected cells.

Original languageEnglish (US)
Pages (from-to)2417-2422
Number of pages6
JournalJournal of General Virology
Volume80
Issue number9
StatePublished - 1999
Externally publishedYes

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Gallid Herpesvirus 3
Human Herpesvirus 1
Genome
Open Reading Frames
Genes
Ribonucleotide Reductases
DNA-Directed DNA Polymerase
Proline
Amino Acid Sequence
Viruses
Phenotype
Messenger RNA

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Identification and transcriptional analysis of the homologues of the herpes simplex virus type 1 UL30 to UL40 genes in the genome of nononcogenic Marek's disease virus serotype 2. / Izumiya, Yoshihiro; Jang, Hyung Kwan; Sugawara, Mie; Ikeda, Yasuhiro H; Miura, Ryuichi; Nishimura, Yorihiro; Nakamura, Kazuya; Miyazawa, Takayuki; Kai, Chieko; Mikami, Takeshi.

In: Journal of General Virology, Vol. 80, No. 9, 1999, p. 2417-2422.

Research output: Contribution to journalArticle

Izumiya, Y, Jang, HK, Sugawara, M, Ikeda, YH, Miura, R, Nishimura, Y, Nakamura, K, Miyazawa, T, Kai, C & Mikami, T 1999, 'Identification and transcriptional analysis of the homologues of the herpes simplex virus type 1 UL30 to UL40 genes in the genome of nononcogenic Marek's disease virus serotype 2', Journal of General Virology, vol. 80, no. 9, pp. 2417-2422.
Izumiya, Yoshihiro ; Jang, Hyung Kwan ; Sugawara, Mie ; Ikeda, Yasuhiro H ; Miura, Ryuichi ; Nishimura, Yorihiro ; Nakamura, Kazuya ; Miyazawa, Takayuki ; Kai, Chieko ; Mikami, Takeshi. / Identification and transcriptional analysis of the homologues of the herpes simplex virus type 1 UL30 to UL40 genes in the genome of nononcogenic Marek's disease virus serotype 2. In: Journal of General Virology. 1999 ; Vol. 80, No. 9. pp. 2417-2422.
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abstract = "Studies on Marek's disease virus serotype 2 (MDV2) are important for understanding the natural nononcogenic phenotype of MDV. This study reports a 27,535 bp nucleotide sequence of part of the MDV2 genome located in the central unique long (U(L)) region. The analysis revealed 11 complete ORFs with high amino acid sequence identities to the products of other alphaherpesviruses. The MDV2 ORFs were arranged collinearly with the prototype sequence of herpes simplex virus type 1, ranging from the UL30 to UL40 genes. Sequences that were particularly well conserved among alphaherpesviruses were the putative functional domain of the DNA polymerase (UL30) and the ribonucleotide reductase large and small subunits (UL39 and UL40). On the other hand, in contrast to oncogenic MDV1, MDV2 did not contain the conserved proline-repeat region in the UL36 homologue. All the genes identified were confirmed to be transcribed as 3'-coterminal mRNAs and/or unique transcripts in virus-infected cells.",
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