Identification and purification of a yeast transcriptional trans-activator. The yeast homolog of the Rous sarcoma virus internal enhancer binding factor

Larry M Karnitz, D. Poon, P. A. Weil, R. Chalkley

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A cis-acting transcriptional activation sequence (IES2) from the Rous sarcoma virus internal enhancer was found to stimulate transcription of a heterologous gene in Saccharomyces cerevisiae. A hamster protein (termed IBF) which binds to IES2 and stimulates transcription in vitro has previously been purified and was found to have a subunit molecular mass of 40,000 (Karnitz, L., Poon, D., Weil, P.A., and Chalkley, R. (1989) Mol. Cell. Biol. 9, 1929-1939). The identification and purification of the yeast homolog of IBF (yIBF) is reported here. Purified yIBF has a subunit molecular mass of 92,000. This protein functions as a trans-activator of transcription in a heterologous HeLa transcription extract in a cis-element sequence-dependent manner in vitro.

Original languageEnglish (US)
Pages (from-to)6131-6138
Number of pages8
JournalJournal of Biological Chemistry
Volume265
Issue number11
StatePublished - 1990
Externally publishedYes

Fingerprint

Rous sarcoma virus
Trans-Activators
Transcription
Viruses
Yeast
Purification
Yeasts
Molecular mass
Cricetinae
Transcriptional Activation
Saccharomyces cerevisiae
Proteins
Genes
Chemical activation
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry

Cite this

Identification and purification of a yeast transcriptional trans-activator. The yeast homolog of the Rous sarcoma virus internal enhancer binding factor. / Karnitz, Larry M; Poon, D.; Weil, P. A.; Chalkley, R.

In: Journal of Biological Chemistry, Vol. 265, No. 11, 1990, p. 6131-6138.

Research output: Contribution to journalArticle

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