Identification and characterization of RHEBL1, a novel member of Ras family, which activates transcriptional activities of NF-kappa B

Jian Yuan, Yuxi Shan, Xinya Chen, Wenwen Tang, Kuntian Luo, Jun Ni, Bo Wan, Long Yu

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The Ras family of small GTPases regulates a wide variety of cellular functions that include cell growth, differentiation, and transformation. In this study, we identified and characterized a novel member of Ras family named RHEBL1, belonging to the Rheb branch of small GTPase proteins. The cDNA sequence contains an open reading frame of 551 bp, encoding a putative protein of 183 amino acid residues. The expression pattern of RHEBL1 showed that it was ubiquitously expressed in 17 tissues. RHEBL1 gene encodes a 20.69 kDa protein, localized in cytoplasm when overexpressed in COS7 cells. Reporter gene assays showed that overexpression of RHEBL1 in HEK 293T cells strongly activated the transcriptional activities of NF-kappa B, while the mutant (D60K) only weakly activates NF-kappa B-mediated transcription. Our findings suggest that RHEBL1 is a positive regulator of NF-kappa B-mediated gene transcription.

Original languageEnglish (US)
Pages (from-to)205-214
Number of pages10
JournalMolecular Biology Reports
Volume32
Issue number4
DOIs
StatePublished - Dec 2005

Keywords

  • G-protein
  • NF-kappa B
  • RHEB
  • RHEBL1
  • Transcription

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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