Identification and characterization of RAD9B, a paralog of the RAD9 checkpoint gene

Vanessa M. Dufault, Andrea J. Oestreich, Benjamin T. Vroman, Larry M Karnitz

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

RAD9 is an integral element of the PCNA-like HUS1-RAD1-RAD9 (9-1-1) complex that participates in genotoxin-induced CHK1 activation. We have identified a novel RAD9 paralog, dubbed RAD9B, in humans and mice. RAD9 and RAD9B share extensive amino acid homology throughout their entire sequences (36% identity, 48% similarity). Northern blotting revealed that RAD9B transcripts are highly expressed in human testes, with lower levels found in skeletal muscle. In contrast, RT-PCR analysis and immunoprecipitation demonstrated that RAD9B is also expressed in tumor cells. Like RAD9, RAD9B associates with HUS1, RAD1, and RAD17, suggesting that it is a RAD9 paralog that engages in similar biochemical reactions. In addition, we have also shown that RAD9 and RAD9B interact with the HUS1 paralog, HUS1B. Taken together, these results suggest that these proteins can combinatorially assemble into distinct 9-1-1 clamps that may have distinct biological functions.

Original languageEnglish (US)
Pages (from-to)644-651
Number of pages8
JournalGenomics
Volume82
Issue number6
DOIs
StatePublished - Dec 2003

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Mutagens
Proliferating Cell Nuclear Antigen
Immunoprecipitation
Northern Blotting
Genes
Testis
Skeletal Muscle
Amino Acids
Polymerase Chain Reaction
Neoplasms
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Identification and characterization of RAD9B, a paralog of the RAD9 checkpoint gene. / Dufault, Vanessa M.; Oestreich, Andrea J.; Vroman, Benjamin T.; Karnitz, Larry M.

In: Genomics, Vol. 82, No. 6, 12.2003, p. 644-651.

Research output: Contribution to journalArticle

Dufault, Vanessa M. ; Oestreich, Andrea J. ; Vroman, Benjamin T. ; Karnitz, Larry M. / Identification and characterization of RAD9B, a paralog of the RAD9 checkpoint gene. In: Genomics. 2003 ; Vol. 82, No. 6. pp. 644-651.
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