Identification and Characterization of Novel, Naturally Processed Measles Virus Class II HLA-DRB1 Peptides

Inna G. Ovsyannikova, Kenneth L. Johnson, David C. Muddiman, Robert A. Vierkant, Gregory A. Poland

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Abstract

Previously, we identified a naturally processed and presented measles virus (MV) 19-amino-acid peptide, ASDVETAEGGEIHELLRLQ (MV-P), derived from the phosphoprotein and eluted from the human leukocyte antigen (HLA) class II molecule by using mass spectrometry. We report here the identification of a 14-amino-acid peptide, SAGKVSSTLASELG, derived from the MV nucleoprotein (MV-N) bound to HLA-DRB1*0301. Peripheral blood mononuclear cells (PBMC) from 281 previously vaccinated measles-mumps-rubella II (MMR-II) subjects (HLA discordant) were studied for peptide recognition by T cells. Significant gamma interferon (IFN-γ) responses to MV-P and MV-N peptides were observed in 55.9 and 15.3% of subjects, respectively. MV-P- and MV-N-specific interleukin-4 (IL-4) responses were detected in 19.2 and 23.1%, respectively, of PBMC samples. Peptide-specific cytokine responses and HLA-DRB1 allele associations revealed that, for the MV-P peptide, the allele with the strongest association with both IFN-γ (P = 0.02) and IL-4 (P = 0.03) secretion was DRB1*0301. For MV-N, the allele with the strongest association with IFN-γ secretion was DRB1*1501 (P = 0.04), and the alleles with the strongest associations with IL-4 secretion were DRB1*1103 and DRB1*1303 (P = 0.01). These results indicate that HLA class II MV proteins can be processed, presented, and identified, and the ability to generate cell-mediated immune responses can be demonstrated. This information is promising for new vaccine design strategies with peptide-based vaccines.

Original languageEnglish (US)
Pages (from-to)42-51
Number of pages10
JournalJournal of Virology
Volume78
Issue number1
DOIs
StatePublished - Jan 2004

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HLA-D Antigens
Measles virus
HLA Antigens
peptides
Peptides
Alleles
Interleukin-4
interleukin-4
alleles
secretion
interferons
mononuclear leukocytes
Interferons
Blood Cells
Peptide T
HLA antigens
Amino Acids
vaccines
Mumps
Subunit Vaccines

ASJC Scopus subject areas

  • Immunology

Cite this

Ovsyannikova, I. G., Johnson, K. L., Muddiman, D. C., Vierkant, R. A., & Poland, G. A. (2004). Identification and Characterization of Novel, Naturally Processed Measles Virus Class II HLA-DRB1 Peptides. Journal of Virology, 78(1), 42-51. https://doi.org/10.1128/JVI.78.1.42-51.2004

Identification and Characterization of Novel, Naturally Processed Measles Virus Class II HLA-DRB1 Peptides. / Ovsyannikova, Inna G.; Johnson, Kenneth L.; Muddiman, David C.; Vierkant, Robert A.; Poland, Gregory A.

In: Journal of Virology, Vol. 78, No. 1, 01.2004, p. 42-51.

Research output: Contribution to journalArticle

Ovsyannikova, Inna G. ; Johnson, Kenneth L. ; Muddiman, David C. ; Vierkant, Robert A. ; Poland, Gregory A. / Identification and Characterization of Novel, Naturally Processed Measles Virus Class II HLA-DRB1 Peptides. In: Journal of Virology. 2004 ; Vol. 78, No. 1. pp. 42-51.
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