Identification and characterisation of SNPs in candidate genes for schizophrenia

N. M. Williams, T. Bowen, G. Spurlock, H. Williams, Nadine Norton, J. Austin, A. Walters, M. J. Owen, M. C. O'Donovan

Research output: Contribution to journalArticle

Abstract

Improvements in molecular genetic technology and the progress of the Human Genome Project now allows us to replace limited candidate gene studies with almost complete systems-based tests of alternative hypotheses of aetiology of psychiatric disorders. As part of a systematic analysis of the glutamatergic and GABAergic hypotheses of schizophrenia we have surveyed over 25 candidate genes for sequence variation. In order to achieve this we have used either their previously deduced genomic structures or the available human genome sequence data to predict the intron/exon boundaries of genes. The 5′and 3′UTR's together with the coding sequence for all genes have been screened for sequence variants using dHPLC heteroduplex analysis in a sample of 14 schizophrenic patients, and the resulting SNP's characterized using Big Dye(tm) sequencing. Thus we have identified a panel of SNP's spanning each candidate gene. As well as being useful for direct candidate gene analysis and indirect LD mapping studies for schizophrenia and other disorders including, epilepsy and Alzheimer's disease, these SNP's are also of potential interest in studying sequence variation in human genes.

Original languageEnglish (US)
Pages (from-to)462
Number of pages1
JournalAmerican Journal of Medical Genetics - Neuropsychiatric Genetics
Volume96
Issue number4
StatePublished - Aug 7 2000
Externally publishedYes

Fingerprint

Single Nucleotide Polymorphism
Schizophrenia
Genes
Heteroduplex Analysis
Human Genome Project
Genetic Association Studies
Human Genome
Introns
Psychiatry
Molecular Biology
Exons
Epilepsy
Alzheimer Disease
Coloring Agents
Technology

ASJC Scopus subject areas

  • Genetics(clinical)
  • Cellular and Molecular Neuroscience
  • Psychiatry and Mental health

Cite this

Williams, N. M., Bowen, T., Spurlock, G., Williams, H., Norton, N., Austin, J., ... O'Donovan, M. C. (2000). Identification and characterisation of SNPs in candidate genes for schizophrenia. American Journal of Medical Genetics - Neuropsychiatric Genetics, 96(4), 462.

Identification and characterisation of SNPs in candidate genes for schizophrenia. / Williams, N. M.; Bowen, T.; Spurlock, G.; Williams, H.; Norton, Nadine; Austin, J.; Walters, A.; Owen, M. J.; O'Donovan, M. C.

In: American Journal of Medical Genetics - Neuropsychiatric Genetics, Vol. 96, No. 4, 07.08.2000, p. 462.

Research output: Contribution to journalArticle

Williams, NM, Bowen, T, Spurlock, G, Williams, H, Norton, N, Austin, J, Walters, A, Owen, MJ & O'Donovan, MC 2000, 'Identification and characterisation of SNPs in candidate genes for schizophrenia', American Journal of Medical Genetics - Neuropsychiatric Genetics, vol. 96, no. 4, pp. 462.
Williams, N. M. ; Bowen, T. ; Spurlock, G. ; Williams, H. ; Norton, Nadine ; Austin, J. ; Walters, A. ; Owen, M. J. ; O'Donovan, M. C. / Identification and characterisation of SNPs in candidate genes for schizophrenia. In: American Journal of Medical Genetics - Neuropsychiatric Genetics. 2000 ; Vol. 96, No. 4. pp. 462.
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