Abstract
Measurement of the Haemoglobin F in red cell haemolysates is important in the diagnosis of δβ thalassaemia, hereditary persistence of fetal haemoglobin (HPFH) and in the diagnosis and management of sickle cell disease. The distribution of Hb F in red cells is useful in the diagnosis of HPFH and in the assessment of feto-maternal haemorrhage. The methods of quantifying Hb F are described together with pitfalls in undertaking these laboratory tests with particular emphasis on automated high-performance liquid chromatography and capillary electrophoresis.
Original language | English (US) |
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Pages (from-to) | 14-20 |
Number of pages | 7 |
Journal | International Journal of Laboratory Hematology |
Volume | 34 |
Issue number | 1 |
DOIs | |
State | Published - Feb 2012 |
Keywords
- Capillary electrophoresis
- Haemoglobin F
- Haemoglobinopathy
- High-performance liquid chromatography
- Recommended methods
- Thalassaemia
ASJC Scopus subject areas
- Hematology
- Clinical Biochemistry
- Biochemistry, medical