I region associated determinants: expression on mitogen stimulated lymphocytes and detection by cytotoxic T cells

M. Nabholz, H. Young, A. Rynbeek, R. Boccardo, C. S. David, T. Meo, V. Miggiano, D. C. Shreffler

Research output: Contribution to journalArticle

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Abstract

We have studied the expression of Ia antigens, controlled by genes in the I region of the H 2 complex, on phytohemagglutinin (PHA) stimulated lymph node cells and on lipopolysaccharide (LPS) stimulated spleen cells, and have compared these two types of cell populations as targets for killer cells in the cell mediated lympholysis (CML) assay. PHA targets are almost completely insensitive to complement mediated lysis by anti Ia sera while the majority of LPS targets are killed. T cell mediated lysis against I region determinants was also detected, and these determinants, in contrast to H 2K and H 2D CML determinants, seem to be much more strongly expressed on LPS stimulated cells. No differences in the kinetics of the response to K or I region CML determinants were observed. Lysis by killer cells can also be obtained against incompatibilities which do not give rise to strong skin graft rejection and against determinants which are most probably controlled by genes outside the K D interval.

Original languageEnglish (US)
Pages (from-to)594-599
Number of pages6
JournalEuropean Journal of Immunology
Volume5
Issue number9
StatePublished - 1975
Externally publishedYes

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Mitogens
Lymphocytes
T-Lymphocytes
Lipopolysaccharides
Phytohemagglutinins
Health Services Needs and Demand
Histocompatibility Antigens Class II
Graft Rejection
Genes
Spleen
Lymph Nodes
Skin
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Nabholz, M., Young, H., Rynbeek, A., Boccardo, R., David, C. S., Meo, T., ... Shreffler, D. C. (1975). I region associated determinants: expression on mitogen stimulated lymphocytes and detection by cytotoxic T cells. European Journal of Immunology, 5(9), 594-599.

I region associated determinants : expression on mitogen stimulated lymphocytes and detection by cytotoxic T cells. / Nabholz, M.; Young, H.; Rynbeek, A.; Boccardo, R.; David, C. S.; Meo, T.; Miggiano, V.; Shreffler, D. C.

In: European Journal of Immunology, Vol. 5, No. 9, 1975, p. 594-599.

Research output: Contribution to journalArticle

Nabholz, M, Young, H, Rynbeek, A, Boccardo, R, David, CS, Meo, T, Miggiano, V & Shreffler, DC 1975, 'I region associated determinants: expression on mitogen stimulated lymphocytes and detection by cytotoxic T cells', European Journal of Immunology, vol. 5, no. 9, pp. 594-599.
Nabholz, M. ; Young, H. ; Rynbeek, A. ; Boccardo, R. ; David, C. S. ; Meo, T. ; Miggiano, V. ; Shreffler, D. C. / I region associated determinants : expression on mitogen stimulated lymphocytes and detection by cytotoxic T cells. In: European Journal of Immunology. 1975 ; Vol. 5, No. 9. pp. 594-599.
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AU - Boccardo, R.

AU - David, C. S.

AU - Meo, T.

AU - Miggiano, V.

AU - Shreffler, D. C.

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