Hypothermia predicts hepatic failure after extensive hepatectomy in mice

Norifumi Ohashi, Akimasa Nakao, Tomohide Hori, Shinji Uemoto, Sura Jermanus, Feng Chen, Justin H Nguyen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

AIM: To investigate the effect of hypothermia on the function of the liver remnant (LR) after extended hepatectomy. METHODS: We performed a 75% partial hepatectomy (PH) in male C57BL/6J mice. Body temperature was measured with a rectal probe. The study mice were prospectively grouped as hypothermic (HT) or normothermic (NT) if their body temperature was <34 ° C vs ≥ 34 ° C , respectively. Blood and liver samples were obtained at 24 and 48 h after 75% PH. Various factors during and after 75% PH were compared at each time point and the most important factor for a good outcome after 75% PH was determined. RESULTS: At 24 and 48 h after 75% PH, LR weight was decreased in HT mice compared with that in NT mice and the assay results in the HT mice were consistent with liver failure. NT mice had normal liver regeneration. Each intra- and post-operative factor which showed statistical significance in univariate analysis was evaluated by multivariate analysis. The most important factor for a good outcome after 75% PH was body temperature at both 24 and 48 h after surgery. CONCLUSION: Hypothermia after an extensive hepatectomy predicts impending liver failure and may be a useful clinical marker for early detection of liver failure after extended hepatectomy.

Original languageEnglish (US)
Pages (from-to)170-181
Number of pages12
JournalWorld Journal of Hepatology
Volume5
Issue number4
DOIs
StatePublished - Apr 2013

Fingerprint

Liver Failure
Hepatectomy
Hypothermia
Body Temperature
Liver
Liver Regeneration
Inbred C57BL Mouse
Multivariate Analysis
Biomarkers
Weights and Measures

Keywords

  • Hepatectomy
  • Hypothermia
  • Liver failure
  • Mortality
  • Predictor

ASJC Scopus subject areas

  • Hepatology

Cite this

Hypothermia predicts hepatic failure after extensive hepatectomy in mice. / Ohashi, Norifumi; Nakao, Akimasa; Hori, Tomohide; Uemoto, Shinji; Jermanus, Sura; Chen, Feng; Nguyen, Justin H.

In: World Journal of Hepatology, Vol. 5, No. 4, 04.2013, p. 170-181.

Research output: Contribution to journalArticle

Ohashi, N, Nakao, A, Hori, T, Uemoto, S, Jermanus, S, Chen, F & Nguyen, JH 2013, 'Hypothermia predicts hepatic failure after extensive hepatectomy in mice', World Journal of Hepatology, vol. 5, no. 4, pp. 170-181. https://doi.org/10.4254/wjh.v5.i4.170
Ohashi, Norifumi ; Nakao, Akimasa ; Hori, Tomohide ; Uemoto, Shinji ; Jermanus, Sura ; Chen, Feng ; Nguyen, Justin H. / Hypothermia predicts hepatic failure after extensive hepatectomy in mice. In: World Journal of Hepatology. 2013 ; Vol. 5, No. 4. pp. 170-181.
@article{4ad486183fff44eb86d7bb6c90e3c8fc,
title = "Hypothermia predicts hepatic failure after extensive hepatectomy in mice",
abstract = "AIM: To investigate the effect of hypothermia on the function of the liver remnant (LR) after extended hepatectomy. METHODS: We performed a 75{\%} partial hepatectomy (PH) in male C57BL/6J mice. Body temperature was measured with a rectal probe. The study mice were prospectively grouped as hypothermic (HT) or normothermic (NT) if their body temperature was <34 ° C vs ≥ 34 ° C , respectively. Blood and liver samples were obtained at 24 and 48 h after 75{\%} PH. Various factors during and after 75{\%} PH were compared at each time point and the most important factor for a good outcome after 75{\%} PH was determined. RESULTS: At 24 and 48 h after 75{\%} PH, LR weight was decreased in HT mice compared with that in NT mice and the assay results in the HT mice were consistent with liver failure. NT mice had normal liver regeneration. Each intra- and post-operative factor which showed statistical significance in univariate analysis was evaluated by multivariate analysis. The most important factor for a good outcome after 75{\%} PH was body temperature at both 24 and 48 h after surgery. CONCLUSION: Hypothermia after an extensive hepatectomy predicts impending liver failure and may be a useful clinical marker for early detection of liver failure after extended hepatectomy.",
keywords = "Hepatectomy, Hypothermia, Liver failure, Mortality, Predictor",
author = "Norifumi Ohashi and Akimasa Nakao and Tomohide Hori and Shinji Uemoto and Sura Jermanus and Feng Chen and Nguyen, {Justin H}",
year = "2013",
month = "4",
doi = "10.4254/wjh.v5.i4.170",
language = "English (US)",
volume = "5",
pages = "170--181",
journal = "World Journal of Hepatology",
issn = "1948-5182",
publisher = "Baishideng Publishing Group",
number = "4",

}

TY - JOUR

T1 - Hypothermia predicts hepatic failure after extensive hepatectomy in mice

AU - Ohashi, Norifumi

AU - Nakao, Akimasa

AU - Hori, Tomohide

AU - Uemoto, Shinji

AU - Jermanus, Sura

AU - Chen, Feng

AU - Nguyen, Justin H

PY - 2013/4

Y1 - 2013/4

N2 - AIM: To investigate the effect of hypothermia on the function of the liver remnant (LR) after extended hepatectomy. METHODS: We performed a 75% partial hepatectomy (PH) in male C57BL/6J mice. Body temperature was measured with a rectal probe. The study mice were prospectively grouped as hypothermic (HT) or normothermic (NT) if their body temperature was <34 ° C vs ≥ 34 ° C , respectively. Blood and liver samples were obtained at 24 and 48 h after 75% PH. Various factors during and after 75% PH were compared at each time point and the most important factor for a good outcome after 75% PH was determined. RESULTS: At 24 and 48 h after 75% PH, LR weight was decreased in HT mice compared with that in NT mice and the assay results in the HT mice were consistent with liver failure. NT mice had normal liver regeneration. Each intra- and post-operative factor which showed statistical significance in univariate analysis was evaluated by multivariate analysis. The most important factor for a good outcome after 75% PH was body temperature at both 24 and 48 h after surgery. CONCLUSION: Hypothermia after an extensive hepatectomy predicts impending liver failure and may be a useful clinical marker for early detection of liver failure after extended hepatectomy.

AB - AIM: To investigate the effect of hypothermia on the function of the liver remnant (LR) after extended hepatectomy. METHODS: We performed a 75% partial hepatectomy (PH) in male C57BL/6J mice. Body temperature was measured with a rectal probe. The study mice were prospectively grouped as hypothermic (HT) or normothermic (NT) if their body temperature was <34 ° C vs ≥ 34 ° C , respectively. Blood and liver samples were obtained at 24 and 48 h after 75% PH. Various factors during and after 75% PH were compared at each time point and the most important factor for a good outcome after 75% PH was determined. RESULTS: At 24 and 48 h after 75% PH, LR weight was decreased in HT mice compared with that in NT mice and the assay results in the HT mice were consistent with liver failure. NT mice had normal liver regeneration. Each intra- and post-operative factor which showed statistical significance in univariate analysis was evaluated by multivariate analysis. The most important factor for a good outcome after 75% PH was body temperature at both 24 and 48 h after surgery. CONCLUSION: Hypothermia after an extensive hepatectomy predicts impending liver failure and may be a useful clinical marker for early detection of liver failure after extended hepatectomy.

KW - Hepatectomy

KW - Hypothermia

KW - Liver failure

KW - Mortality

KW - Predictor

UR - http://www.scopus.com/inward/record.url?scp=84879149080&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879149080&partnerID=8YFLogxK

U2 - 10.4254/wjh.v5.i4.170

DO - 10.4254/wjh.v5.i4.170

M3 - Article

VL - 5

SP - 170

EP - 181

JO - World Journal of Hepatology

JF - World Journal of Hepatology

SN - 1948-5182

IS - 4

ER -