Hypomagnesemia inhibits nitric oxide release from coronary endothelium

Protective role of magnesium infusion after cardiac operations

Paul J. Pearson, Paulo R B Evora, John F. Seccombe, Hartzell V Schaff

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Background. Postoperative hypomagnesemia is common in patients who have undergone cardiac operations and is associated with clinically significant morbidity resulting from atrial and ventricular dysrhythmias. Magnesium supplementation may increase the cardiac index in the early postoperative period. Methods. The action of the magnesium cation on coronary vascular reactivity was studied. Segments of canine epicardial coronary artery were suspended in organ chambers to measure isometric force (95% O2/5% CO2, 37°C). Results. In coronary segments constricted with prostaglandin F(2α) (2 x 10-06 mol/L), acetylcholine and adenosine diphosphate (10-9 to 10- 4 mol/L) induced vasodilation in arteries with endothelium (n = 10, each group; p < 0.05). Acetylcholine-mediated vasodilation was blocked by N(G)- monomethyl-L-arginine (10-4 mol/L) and N(G)-nitro-L-arginine (10-4 mol/L), two inhibitors of nitric oxide synthesis from L-arginine (n = 10, p < 0.05). The removal of magnesium from the organ chamber solution impaired vasodilation in response to acetylcholine and adenosine diphosphate. However, normal endothelium-dependent vasodilation could be restored by return of magnesium to the bathing solution. Vascular relaxation in response to bradykinin (10-9 to 10-6 mol/L), which was found to induce endothelium- dependent vasodilation independent of nitric oxide production, was unaffected by magnesium removal (n = 10). Conclusions. Hypomagnesemia selectively impaired the release of nitric oxide from the coronary endothelium. Because nitric oxide is a potent endogenous nitro-vasodilator and inhibitor of platelet aggregation and adhesion, hypomagnesemia could promote vasoconstriction and coronary thrombosis in the early postoperative period.

Original languageEnglish (US)
Pages (from-to)967-972
Number of pages6
JournalAnnals of Thoracic Surgery
Volume65
Issue number4
DOIs
StatePublished - 1998

Fingerprint

Vasodilation
Magnesium
Endothelium
Nitric Oxide
Acetylcholine
Arginine
Postoperative Period
Adenosine Diphosphate
Blood Vessels
Coronary Thrombosis
Platelet Aggregation Inhibitors
Prostaglandins F
Bradykinin
Vasoconstriction
Vasodilator Agents
Cations
Canidae
Coronary Vessels
Arteries
Morbidity

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

Cite this

Hypomagnesemia inhibits nitric oxide release from coronary endothelium : Protective role of magnesium infusion after cardiac operations. / Pearson, Paul J.; Evora, Paulo R B; Seccombe, John F.; Schaff, Hartzell V.

In: Annals of Thoracic Surgery, Vol. 65, No. 4, 1998, p. 967-972.

Research output: Contribution to journalArticle

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abstract = "Background. Postoperative hypomagnesemia is common in patients who have undergone cardiac operations and is associated with clinically significant morbidity resulting from atrial and ventricular dysrhythmias. Magnesium supplementation may increase the cardiac index in the early postoperative period. Methods. The action of the magnesium cation on coronary vascular reactivity was studied. Segments of canine epicardial coronary artery were suspended in organ chambers to measure isometric force (95{\%} O2/5{\%} CO2, 37°C). Results. In coronary segments constricted with prostaglandin F(2α) (2 x 10-06 mol/L), acetylcholine and adenosine diphosphate (10-9 to 10- 4 mol/L) induced vasodilation in arteries with endothelium (n = 10, each group; p < 0.05). Acetylcholine-mediated vasodilation was blocked by N(G)- monomethyl-L-arginine (10-4 mol/L) and N(G)-nitro-L-arginine (10-4 mol/L), two inhibitors of nitric oxide synthesis from L-arginine (n = 10, p < 0.05). The removal of magnesium from the organ chamber solution impaired vasodilation in response to acetylcholine and adenosine diphosphate. However, normal endothelium-dependent vasodilation could be restored by return of magnesium to the bathing solution. Vascular relaxation in response to bradykinin (10-9 to 10-6 mol/L), which was found to induce endothelium- dependent vasodilation independent of nitric oxide production, was unaffected by magnesium removal (n = 10). Conclusions. Hypomagnesemia selectively impaired the release of nitric oxide from the coronary endothelium. Because nitric oxide is a potent endogenous nitro-vasodilator and inhibitor of platelet aggregation and adhesion, hypomagnesemia could promote vasoconstriction and coronary thrombosis in the early postoperative period.",
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