Hypochlorous-Acid-Generating Electrochemical Scaffold for Treatment of Wound Biofilms

Mia Mae Kiamco, Hannah M. Zmuda, Abdelrhman Mohamed, Douglas R. Call, Yash S. Raval, Robin Patel, Haluk Beyenal

Research output: Contribution to journalArticle

Abstract

Biofilm formation causes prolonged wound infections due to the dense biofilm structure, differential gene regulation to combat stress, and production of extracellular polymeric substances. Acinetobacter baumannii, Staphylococcus aureus, and Pseudomonas aeruginosa are three difficult-to-treat biofilm-forming bacteria frequently found in wound infections. This work describes a novel wound dressing in the form of an electrochemical scaffold (e-scaffold) that generates controlled, low concentrations of hypochlorous acid (HOCl) suitable for killing biofilm communities without substantially damaging host tissue. Production of HOCl near the e-scaffold surface was verified by measuring its concentration using needle-type microelectrodes. E-scaffolds producing 17, 10 and 7 mM HOCl completely eradicated S. aureus, A. baumannii, and P. aeruginosa biofilms after 3 hours, 2 hours, and 1 hour, respectively. Cytotoxicity and histopathological assessment showed no discernible harm to host tissues when e-scaffolds were applied to explant biofilms. The described strategy may provide a novel antibiotic-free strategy for treating persistent biofilm-associated infections, such as wound infections.

Original languageEnglish (US)
Article number2683
JournalScientific reports
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2019

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Hypochlorous Acid
Biofilms
Wounds and Injuries
Wound Infection
Acinetobacter baumannii
Pseudomonas aeruginosa
Staphylococcus aureus
Microelectrodes
Bandages
Needles
Anti-Bacterial Agents
Bacteria
Infection

ASJC Scopus subject areas

  • General

Cite this

Kiamco, M. M., Zmuda, H. M., Mohamed, A., Call, D. R., Raval, Y. S., Patel, R., & Beyenal, H. (2019). Hypochlorous-Acid-Generating Electrochemical Scaffold for Treatment of Wound Biofilms. Scientific reports, 9(1), [2683]. https://doi.org/10.1038/s41598-019-38968-y

Hypochlorous-Acid-Generating Electrochemical Scaffold for Treatment of Wound Biofilms. / Kiamco, Mia Mae; Zmuda, Hannah M.; Mohamed, Abdelrhman; Call, Douglas R.; Raval, Yash S.; Patel, Robin; Beyenal, Haluk.

In: Scientific reports, Vol. 9, No. 1, 2683, 01.12.2019.

Research output: Contribution to journalArticle

Kiamco, Mia Mae ; Zmuda, Hannah M. ; Mohamed, Abdelrhman ; Call, Douglas R. ; Raval, Yash S. ; Patel, Robin ; Beyenal, Haluk. / Hypochlorous-Acid-Generating Electrochemical Scaffold for Treatment of Wound Biofilms. In: Scientific reports. 2019 ; Vol. 9, No. 1.
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