Hydrogen/deuterium exchange reflects binding of human centrin 2 to Ca 2+ and Xeroderma pigmentosum Group C peptide: An example of EX1 kinetics

Justin B. Sperry, Zachary C. Ryan, Rajiv Kumar, Michael L. Gross

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Xeroderma pigmentosum (XP) is a genetic disease affecting 1 in 10,000-100,000 and predisposes people to early-age skin cancer, a disease that is increasing. Those with XP have decreased ability to repair UV-induced DNA damage, leading to increased susceptibility of cancerous non-melanomas and melanomas. A vital, heterotrimeric protein complex is linked to the nucleotide excision repair pathway for the damaged DNA. The complex consists of XPC protein, human centrin 2, and RAD23B. One of the members, human centrin 2, is a ubiquitous, acidic, Ca2+-binding protein belonging to the calmodulin superfamily. The XPC protein contains a sequence motif specific for binding to human centrin 2. We report here the Ca2+-binding properties of human centrin 2 and its interaction with the XPC peptide motif. We utilized a region-specific H/D exchange protocol to localize the interaction of the XPC peptide with the C-terminal domain of centrin, the binding of which is different than that of calmodulin complexes. The binding dynamics of human centrin 2 to the XPC peptide in the absence and presence of Ca2+ are revealed by the observation of EX1 H/D exchange regime, indicating that a locally unfolded population exists in solution and undergoes fast H/D exchange.

Original languageEnglish (US)
Pages (from-to)302-309
Number of pages8
JournalInternational Journal of Mass Spectrometry
Volume330-332
DOIs
StatePublished - Dec 15 2012

Fingerprint

C-Peptide
Deuterium
Calmodulin
Peptides
peptides
deuterium
Hydrogen
Proteins
Kinetics
calmodulin
kinetics
proteins
DNA
Repair
hydrogen
Nucleotides
deoxyribonucleic acid
Ion exchange
Skin
Sulfamethoxazole Drug Combination Trimethoprim

Keywords

  • Centrin 2
  • EX1 kinetic
  • Hydrogen/deuterium exchange
  • Protein
  • Protein/peptide interaction
  • Xeroderma pigmentosum

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Spectroscopy
  • Condensed Matter Physics
  • Instrumentation

Cite this

Hydrogen/deuterium exchange reflects binding of human centrin 2 to Ca 2+ and Xeroderma pigmentosum Group C peptide : An example of EX1 kinetics. / Sperry, Justin B.; Ryan, Zachary C.; Kumar, Rajiv; Gross, Michael L.

In: International Journal of Mass Spectrometry, Vol. 330-332, 15.12.2012, p. 302-309.

Research output: Contribution to journalArticle

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abstract = "Xeroderma pigmentosum (XP) is a genetic disease affecting 1 in 10,000-100,000 and predisposes people to early-age skin cancer, a disease that is increasing. Those with XP have decreased ability to repair UV-induced DNA damage, leading to increased susceptibility of cancerous non-melanomas and melanomas. A vital, heterotrimeric protein complex is linked to the nucleotide excision repair pathway for the damaged DNA. The complex consists of XPC protein, human centrin 2, and RAD23B. One of the members, human centrin 2, is a ubiquitous, acidic, Ca2+-binding protein belonging to the calmodulin superfamily. The XPC protein contains a sequence motif specific for binding to human centrin 2. We report here the Ca2+-binding properties of human centrin 2 and its interaction with the XPC peptide motif. We utilized a region-specific H/D exchange protocol to localize the interaction of the XPC peptide with the C-terminal domain of centrin, the binding of which is different than that of calmodulin complexes. The binding dynamics of human centrin 2 to the XPC peptide in the absence and presence of Ca2+ are revealed by the observation of EX1 H/D exchange regime, indicating that a locally unfolded population exists in solution and undergoes fast H/D exchange.",
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