Hydrogen-Deuterium Exchange Mass Spectrometry Reveals Calcium Binding Properties and Allosteric Regulation of Downstream Regulatory Element Antagonist Modulator (DREAM)

Jun Zhang, Jing Li, Theodore A. Craig, Rajiv Kumar, Michael L. Gross

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Downstream regulatory element antagonist modulator (DREAM) is an EF-hand Ca2+-binding protein that also binds to a specific DNA sequence, downstream regulatory elements (DRE), and thereby regulates transcription in a calcium-dependent fashion. DREAM binds to DRE in the absence of Ca2+ but detaches from DRE under Ca2+ stimulation, allowing gene expression. The Ca2+ binding properties of DREAM and the consequences of the binding on protein structure are key to understanding the function of DREAM. Here we describe the application of hydrogen-deuterium exchange mass spectrometry (HDX-MS) and site-directed mutagenesis to investigate the Ca2+ binding properties and the subsequent conformational changes of full-length DREAM. We demonstrate that all EF-hands undergo large conformation changes upon calcium binding even though the EF-1 hand is not capable of binding to Ca2+. Moreover, EF-2 is a lower-affinity site compared to EF-3 and -4 hands. Comparison of HDX profiles between wild-type DREAM and two EF-1 mutated constructs illustrates that the conformational changes in the EF-1 hand are induced by long-range structural interactions. HDX analyses also reveal a conformational change in an N-terminal leucine-charged residue-rich domain (LCD) remote from Ca2+-binding EF-hands. This LCD domain is responsible for the direct interaction between DREAM and cAMP response element-binding protein (CREB) and regulates the recruitment of the co-activator, CREB-binding protein. These long-range interactions strongly suggest how conformational changes transmit the Ca2+ signal to CREB-mediated gene transcription.

Original languageEnglish (US)
Pages (from-to)3523-3530
Number of pages8
JournalBiochemistry
Volume56
Issue number28
DOIs
StatePublished - Jul 18 2017

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Kv Channel-Interacting Proteins
Allosteric Regulation
Deuterium
EF Hand Motifs
Mass spectrometry
Hydrogen
Mass Spectrometry
Calcium
Peptide Elongation Factor 1
Cyclic AMP Response Element-Binding Protein
Carrier Proteins
Transcription
Liquid crystal displays
Peptide Elongation Factor 2
Mutagenesis
DNA sequences
Site-Directed Mutagenesis
Protein Binding
Gene expression
Leucine

ASJC Scopus subject areas

  • Biochemistry

Cite this

Hydrogen-Deuterium Exchange Mass Spectrometry Reveals Calcium Binding Properties and Allosteric Regulation of Downstream Regulatory Element Antagonist Modulator (DREAM). / Zhang, Jun; Li, Jing; Craig, Theodore A.; Kumar, Rajiv; Gross, Michael L.

In: Biochemistry, Vol. 56, No. 28, 18.07.2017, p. 3523-3530.

Research output: Contribution to journalArticle

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