Human telomerase reverse transcriptase (hTERT) gene expression in FNA samples from thyroid neoplasms

Martha A. Zeiger, Robert C. Smallridge, Douglas P. Clark, Chien Ko Liang, Sally E. Carty, Charles G. Watson, Robert Udelsman, Motoyasu Saji

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49 Scopus citations

Abstract

Background. Although fine-needle aspiration (FNA) is the most sensitive method for the detection of thyroid carcinoma, it cannot provide a definitive diagnosis of malignancy in 60% of the patients operated on for suspicious lesions. Recently, human telomerase reverse transcriptase (hTERT) has been found to be a diagnostic marker of malignancy. We therefore sought to determine whether hTERT gene expression could serve as an adjunct to FNA in the differential diagnosis of thyroid nodules. Methods. Twenty-four FNA samples from thyroid nodules that were suspected of malignancy were collected. RNA was extracted, and hTERT gene expression was examined by RT- PCR. Cytologic and histologic examinations were also performed. Results. Two of three follicular, three of three Hurthle cell, and eight of eight papillary thyroid carcinomas had corresponding FNA samples that were positive for hTERT. One of two Hurthle cell adenomas was hTERT positive. FNA samples from three follicular adenomas and five hyperplastic nodules were negative for hTERT. Positive and negative predictive values were 93% and 90%, respectively. Conclusions. The detection of hTERT gene expression in thyroid FNA samples holds promise as a diagnostic marker in the distinction of benign from malignant thyroid lesions. Its application could alter the surgical management of these patients.

Original languageEnglish (US)
Pages (from-to)1195-1199
Number of pages5
JournalSurgery
Volume126
Issue number6
DOIs
StatePublished - Jan 1 1999

ASJC Scopus subject areas

  • Surgery

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    Zeiger, M. A., Smallridge, R. C., Clark, D. P., Liang, C. K., Carty, S. E., Watson, C. G., Udelsman, R., & Saji, M. (1999). Human telomerase reverse transcriptase (hTERT) gene expression in FNA samples from thyroid neoplasms. Surgery, 126(6), 1195-1199. https://doi.org/10.1067/msy.2099.101374