Human serum dopamine β hydroxylase: correlation of enzymatic activity with immunoreactive protein in genetically defined samples

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28 Citations (Scopus)

Abstract

An antibody against human adrenal dopamine β hydroxylase (DBH) was used to quantitate immunoreactive DBH protein in human serum by an immunoprecipitation technique. A significant correlation was found between DBH enzyme activity and immunoreactive DBH protein in randomly selected serum samples (r=0.94; N=38; p<.001). Studies of sera from obligate heterozygotes and individuals homozygous for the allele responsible for very low serum DBH enzymatic activity were compatible with a genetically mediated decrease in the quantity of circulating DBH protein in these subjects.

Original languageEnglish (US)
Pages (from-to)155-166
Number of pages12
JournalAmerican Journal of Human Genetics
Volume28
Issue number2
StatePublished - 1976

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Mixed Function Oxygenases
Dopamine
Serum
Proteins
Heterozygote
Immunoprecipitation
Alleles
Antibodies
Enzymes

ASJC Scopus subject areas

  • Genetics

Cite this

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abstract = "An antibody against human adrenal dopamine β hydroxylase (DBH) was used to quantitate immunoreactive DBH protein in human serum by an immunoprecipitation technique. A significant correlation was found between DBH enzyme activity and immunoreactive DBH protein in randomly selected serum samples (r=0.94; N=38; p<.001). Studies of sera from obligate heterozygotes and individuals homozygous for the allele responsible for very low serum DBH enzymatic activity were compatible with a genetically mediated decrease in the quantity of circulating DBH protein in these subjects.",
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