TY - JOUR
T1 - Human platelets contain estrogen receptor α, caveolin-1 and estrogen receptor associated proteins
AU - Jayachandran, Muthuvel
AU - Miller, Virginia M.
N1 - Funding Information:
This work was supported by National Institutes of Health grant HL51736.
PY - 2003/3
Y1 - 2003/3
N2 - Venous thrombosis is a risk factor for oral estrogen treatment in men and women. Platelet responses mediated through estrogen receptors may be involved in these processes. However, expression of estrogen receptors and estrogen receptor-associated protein have not been defined clearly in human platelets. Therefore, the present study was designed to characterize estrogen receptors and estrogen receptor-associated proteins in human platelets. Platelets were obtained from normal female and male blood donors between the ages of 25 and 65 years. Estrogen receptors (ERα and ERβ), heat shock proteins (hsp70 and hsp90), endothelial nitric oxide synthases (eNOS), caveolin-1, -2 and -3 and calmodulin were analyzed in total platelet lysate by immunoblotting. Expression of ERα and ERβ, hsp70, hsp90, eNOS, calmodulin, and caveolin-1 were observed in both sexes. Expression of all proteins was similar in platelets from males and females. Results of the present study suggest for the first time that both estrogen receptors α and β, caveolin-1 and other proteins associated with estrogen receptors and production of nitric oxide are present in platelets of both males and females.
AB - Venous thrombosis is a risk factor for oral estrogen treatment in men and women. Platelet responses mediated through estrogen receptors may be involved in these processes. However, expression of estrogen receptors and estrogen receptor-associated protein have not been defined clearly in human platelets. Therefore, the present study was designed to characterize estrogen receptors and estrogen receptor-associated proteins in human platelets. Platelets were obtained from normal female and male blood donors between the ages of 25 and 65 years. Estrogen receptors (ERα and ERβ), heat shock proteins (hsp70 and hsp90), endothelial nitric oxide synthases (eNOS), caveolin-1, -2 and -3 and calmodulin were analyzed in total platelet lysate by immunoblotting. Expression of ERα and ERβ, hsp70, hsp90, eNOS, calmodulin, and caveolin-1 were observed in both sexes. Expression of all proteins was similar in platelets from males and females. Results of the present study suggest for the first time that both estrogen receptors α and β, caveolin-1 and other proteins associated with estrogen receptors and production of nitric oxide are present in platelets of both males and females.
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U2 - 10.1080/0953710031000080562
DO - 10.1080/0953710031000080562
M3 - Article
C2 - 12745449
AN - SCOPUS:0037365758
SN - 0953-7104
VL - 14
SP - 75
EP - 81
JO - Platelets
JF - Platelets
IS - 2
ER -