Human platelets contain estrogen receptor α, caveolin-1 and estrogen receptor associated proteins

Muthuvel Jayachandran, Virginia M Miller

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Venous thrombosis is a risk factor for oral estrogen treatment in men and women. Platelet responses mediated through estrogen receptors may be involved in these processes. However, expression of estrogen receptors and estrogen receptor-associated protein have not been defined clearly in human platelets. Therefore, the present study was designed to characterize estrogen receptors and estrogen receptor-associated proteins in human platelets. Platelets were obtained from normal female and male blood donors between the ages of 25 and 65 years. Estrogen receptors (ERα and ERβ), heat shock proteins (hsp70 and hsp90), endothelial nitric oxide synthases (eNOS), caveolin-1, -2 and -3 and calmodulin were analyzed in total platelet lysate by immunoblotting. Expression of ERα and ERβ, hsp70, hsp90, eNOS, calmodulin, and caveolin-1 were observed in both sexes. Expression of all proteins was similar in platelets from males and females. Results of the present study suggest for the first time that both estrogen receptors α and β, caveolin-1 and other proteins associated with estrogen receptors and production of nitric oxide are present in platelets of both males and females.

Original languageEnglish (US)
Pages (from-to)75-81
Number of pages7
JournalPlatelets
Volume14
Issue number2
DOIs
StatePublished - Mar 2003

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Caveolin 1
Estrogen Receptor alpha
Blood Platelets
Estrogen Receptors
Nitric Oxide Synthase Type III
Calmodulin
Caveolin 2
Heat-Shock Proteins
estrophilin
Blood Donors
Immunoblotting
Venous Thrombosis
Nitric Oxide
Estrogens
Proteins

ASJC Scopus subject areas

  • Hematology
  • Cell Biology

Cite this

Human platelets contain estrogen receptor α, caveolin-1 and estrogen receptor associated proteins. / Jayachandran, Muthuvel; Miller, Virginia M.

In: Platelets, Vol. 14, No. 2, 03.2003, p. 75-81.

Research output: Contribution to journalArticle

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