TY - JOUR
T1 - Human peripheral nerve macrophages in normal and pathological conditions
AU - Bonetti, Bruno
AU - Monaco, Salvatore
AU - Giannini, Caterina
AU - Ferrari, Sergio
AU - Zanusso, Gian Luigi
AU - Rizzuto, Nicola
N1 - Funding Information:
Acknowledgements We thank S. Galiazzo-Rizzuto for skilled assis-tence with immune electron microscopy, Drs. Giovanni Di Perri, Paola Tonin and Tiziana Cavallaro for critically reading the manuscript, and Prof. Roberto Accolla for generously supplying the monoclonal antibody D 1.12. This work was supported in part by a grant from Associazione ltaliana Sclerosi Multipla to Dr. S. Monaco.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1993/9
Y1 - 1993/9
N2 - We investigated, by immunocytochemistry and immune electron microscopy, the immunophenotype, morphology and functional properties of human peripheral nervous system (PNS) macrophages (MΦ) under normal and pathological conditions. Endoneurial MΦ disclosed an elongated, ramified morphology, with the main processes oriented along the major axis of nerve fibers; they shared several lineage-related and functional markers with monocyte/macrophages and central nervous system (CNS) microglia, including CD4, CR3, CR4 and FcRIII. In addition, basal expression of HLA-DR antigens was exclusively confined to MΦ in normal PNS. In the course of unrelated pathological conditions, resident MΦ underwent activation with transformation to hypertrophic cells or foamy phagocytes and up-regulation of the markers expressed in normal conditions; new expression of a macrophagic antigen was detected on activated MΦ. In different neuropathies, HLA-DR expression was also detected on non-myelin forming Schwann cells with ultrastructural features indicative of denervation. The present results demonstrate that the human PNS is provided with an intrinsic population of immunocompetent and potentially phagocytic MΦ, which represent the peripheral counterpart of CNS microglia.
AB - We investigated, by immunocytochemistry and immune electron microscopy, the immunophenotype, morphology and functional properties of human peripheral nervous system (PNS) macrophages (MΦ) under normal and pathological conditions. Endoneurial MΦ disclosed an elongated, ramified morphology, with the main processes oriented along the major axis of nerve fibers; they shared several lineage-related and functional markers with monocyte/macrophages and central nervous system (CNS) microglia, including CD4, CR3, CR4 and FcRIII. In addition, basal expression of HLA-DR antigens was exclusively confined to MΦ in normal PNS. In the course of unrelated pathological conditions, resident MΦ underwent activation with transformation to hypertrophic cells or foamy phagocytes and up-regulation of the markers expressed in normal conditions; new expression of a macrophagic antigen was detected on activated MΦ. In different neuropathies, HLA-DR expression was also detected on non-myelin forming Schwann cells with ultrastructural features indicative of denervation. The present results demonstrate that the human PNS is provided with an intrinsic population of immunocompetent and potentially phagocytic MΦ, which represent the peripheral counterpart of CNS microglia.
KW - (Human)
KW - Complement receptors
KW - HLA-DR expression
KW - HLA-DR-positive macrophages
KW - Immune electron microscopy
KW - Immunocytochemistry
KW - Peripheral nervous system
KW - Peripheral neuropathy
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U2 - 10.1016/0022-510X(93)90105-8
DO - 10.1016/0022-510X(93)90105-8
M3 - Article
C2 - 8229064
AN - SCOPUS:0027182343
SN - 0022-510X
VL - 118
SP - 158
EP - 168
JO - Journal of the neurological sciences
JF - Journal of the neurological sciences
IS - 2
ER -