Human papillomavirus type 16 integrations in cervical tumors frequently occur in common fragile sites

Erik C Thorland, S. L. Myers, D. H. Persing, G. Sarkar, R. M. McGovern, B. S. Gostout, David I Smith

Research output: Contribution to journalArticle

154 Citations (Scopus)

Abstract

The development of cervical cancer is highly associated with human papillomavirus (HPV) infection. HPV integration into the genome of infected cervical cells is temporally associated with the acquisition of the malignant phenotype. A relationship between the sites of HPV integration in cervical cancer and the position of the common fragile sites (CFSs) has been observed at the cytogenetic level. To explore this relationship at the molecular level, we used a PCR-based method to rapidly isolate cellular sequences flanking the sites of HPV16 integrations in primary cervical tumors. Human bacterial artificial chromosome clones were isolated based on these flanking sequences and used as probes for fluorescence in situ hybridization on metaphases derived from cells cultured in the presence of aphidicolin. Our data demonstrate that HPV16 integrations in cervical tumors frequently occur within CFSs at the molecular level. In addition, we have determined the precise molecular locations of the CFSs FRA6C and FRA17B.

Original languageEnglish (US)
Pages (from-to)5916-5921
Number of pages6
JournalCancer Research
Volume60
Issue number21
StatePublished - Nov 1 2000

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Human papillomavirus 16
Uterine Cervical Neoplasms
Human Artificial Chromosomes
Aphidicolin
Bacterial Artificial Chromosomes
Neoplasms
Papillomavirus Infections
Metaphase
Fluorescence In Situ Hybridization
Cytogenetics
Cultured Cells
Clone Cells
Genome
Phenotype
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Thorland, E. C., Myers, S. L., Persing, D. H., Sarkar, G., McGovern, R. M., Gostout, B. S., & Smith, D. I. (2000). Human papillomavirus type 16 integrations in cervical tumors frequently occur in common fragile sites. Cancer Research, 60(21), 5916-5921.

Human papillomavirus type 16 integrations in cervical tumors frequently occur in common fragile sites. / Thorland, Erik C; Myers, S. L.; Persing, D. H.; Sarkar, G.; McGovern, R. M.; Gostout, B. S.; Smith, David I.

In: Cancer Research, Vol. 60, No. 21, 01.11.2000, p. 5916-5921.

Research output: Contribution to journalArticle

Thorland, EC, Myers, SL, Persing, DH, Sarkar, G, McGovern, RM, Gostout, BS & Smith, DI 2000, 'Human papillomavirus type 16 integrations in cervical tumors frequently occur in common fragile sites', Cancer Research, vol. 60, no. 21, pp. 5916-5921.
Thorland EC, Myers SL, Persing DH, Sarkar G, McGovern RM, Gostout BS et al. Human papillomavirus type 16 integrations in cervical tumors frequently occur in common fragile sites. Cancer Research. 2000 Nov 1;60(21):5916-5921.
Thorland, Erik C ; Myers, S. L. ; Persing, D. H. ; Sarkar, G. ; McGovern, R. M. ; Gostout, B. S. ; Smith, David I. / Human papillomavirus type 16 integrations in cervical tumors frequently occur in common fragile sites. In: Cancer Research. 2000 ; Vol. 60, No. 21. pp. 5916-5921.
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